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Anti E2F1 p-Ser364 monoclonal antibody (clone : #2)

Background

E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor.  E2F1 is regulated during cell cycle progression.  It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage, stabilized, mobilized to nucleus and activated as a transcription factor .  It induces apoptosis by activating transcription of the p53 homolog, p73 (3).  E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.

Data Link  UniProtKB/Swiss-Prot Q01094 (E2F1_HUMAN)

Application

1) Western blotting (-1 ug/ml)
2) ELISA

Not tested for other applications.


Identification of E2F1 protein phosphorylated at p-Ser364 with monoclonal antibody (#2).
MCF cells were grown in the absence (lane 1) or in the presence of etoposide at 10 μM for 16 h (lanes 2 & 3). Crude lysates were prepared and analyzed by Western blotting (lane 3) with the antibody #2 or immunoprecipitated by pantropic anti-E2F1 antibody followed by Western blotting with the antibody #2

References

1. Trimarchi JM & Lees JA ”Sibling rivalry in the E2F family Nat Rev Mol Cell Biol 3:11-20(2002) PMID: 11823794
2. Stevens C et al “Chk2 activates E2F-1 in response to DNA damage”Nat Cell Bio1 5:401-409 (2003) PMID: 12717439
3. Irwin M et al “Role for the p53 homologue p73 in E2F-1- induced apoptosis”Nature 407:645-648 (2000) PMID: 11034215

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1. Trimarchi JM & Lees JA ”Sibling rivalry in the E2F family Nat Rev Mol Cell Biol 3:11-20(2002) PMID: 11823794 2. Stevens C et al “Chk2 activates E2F-1 in response to DNA damage”Nat Cell Bio1 5:401-409 (2003) PMID: 12717439 3. Irwin M et al “Role for the

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.