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Tag proteins antibody

Anti GST (Glutathione S transferase) polyclonal antibody

Background

Glutathione S transferase (GST) from Schistosoma japonicum is commonly used to create fusion proteins. GST-tag has the size of 220 amino acids (roughly 26kDa) and is fused to the N-terminus of a protein. GST fusion proteins can be produced in Escherichia coli, as recombinant proteins and are used to purify and detect proteins of interest. The GST part binds its substrate, glutathione. GST-fusions protein can be easily purified from cell extracts by affinity chromatography with glutathione resin.

Application

1) Western blotting (dilution: 1/2,000 - 1/10,000)
2) Immunoprecipitation (Individual use)
3) ELISA


Fig.1  Detection of GST-tagged protein by Western blotting.
- : Lysate of 293T cells transfected with an empty vector
+ : Lysate of 293T cells transfected with the plasmid carrying the GST-tagged importin gene


Fig.2 Immunoprecipitation of GST-tagged protein by Western blotting.
- : Lysate of 293T cells transfected with an empty vector
+
: Lysate of 293T cells transfected with the plasmid carrying the GST-tagged INbox gene

<Reference>
1. Smith DB & Johnson KS (1988) “Single-step purification of polypeptides expressed in Escherichia coli as fusions of glutathione-S-transferase.” Gene 67:31-40 PMID: 3047011

2. Kaelin WG Jr et al (1991) “Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.” Cell 64:521-532 PMID: 1825028

3. Molecular Cloning: A laboratory Manual (eds. Sambrook,J., Russell,D.W. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 2001) pp.15.36-15.39, pp.18.48-18.59.

Product List

Product Name Cat# Quantity Price

Anti GST

BAM-60-021-EX 100UL

¥ 22,000
$ 294
€ 220

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.