cMyc is a proto-oncogene, which is overexpressed in a wide range of human cancers. Myc gene encodes a transcription factor that regulates a great number of genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferase. It can also act as a transcriptional repressor. It regulates cell growth, apoptosis, differentiation and stem cell self-renewal. Previous studies on the phosphorylation of c-Myc have suggested functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK), cell proliferation and cell cycle regulation. Phosphorylation at Ser62 is required for Ras-induced stabilization and is prerequisite for phosphorylation at Thr58 for its degradation.
Data Link UniProtKB/Swiss-Prot P01106 (MYC_HUMAN)
1) Western blotting (-1ug/ml)
3) Other applications were not tested.
Identification of cMyc protein whose Ser62 is phosphorylated by Western blotting.
Samples : Crude cell extracts of AGS (gastric adenocarcinoma) cells.
Scr : scrambled siRNA was introduced into the cells as a negative control.
Neg.Contol : Negative control siRNA from Qiagen was transfected.
Myc1: siRNA for cMyc was transfected.The data was provided by Drs.A. Khanna and J. Westermark of University of Tampere.
# User's comment; "It certainly looks that S62-p-Myc antibody specifically recognizes c-Myc protein in human cancer cells and will be a very useful resource for future studies."
Dr. Jukka Westermark, Institute of Medical Technology, University of Tampere
This product was used in references 2 and 3.
1. Sears R et al “Multiple Ras-dependent phosphorylation pathways regulate Myc protein stability” Genes Dev 14:2501-2514 (2000) PMID: 11018017
2. Junttila MR et al “CIP2A inhibits PP2A in human malignancies” Cell 130: 51-62 (2007) PMID: 17632056
3. Khanna A et al “MYC-dependent regulation and prognostic role of CIP2A in gastric cancer”J Natl Cancer Inst 101: 793-805 (2009) PMID: 19470954
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.