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useful for the research of a critical role in the differentiation of various cell types

Anti Mitf (Microphthalmia-associated transcription factor) polyclonal antibody

Background

Mitf (Microphthalmia-associated transcription factor) is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It plays a critical role in the differentiation of various cell types such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mutations in Mitf cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome.
Mitf consists of many isoforms that differ in their N-terminal sequences owing to different promoter choices and/or in their internal sequences caused by alternative splicing.

 Data Link : UniProtKB/Swiss-Prot human: O75030(MITF_HUMAN), mouse: Q08874(MITF_MOUSE), chicken: O73871(O73871_CHICK), Xenopus: A4IID0(A4IID0_XENTR), OMIM (human): 156845

Application

1. Western blotting (1/5,000:Different splicing isoforms detected).
2. Immunohistochemistry (dilution: 1/500 ~ 1/1,000)
3. Immunocytochemistry
4. ChIP (chromatin immunoprecipitation) (1/200: Users should examine the best conditions which depend on samples and the ways of extract preparation)


Fig. 1 Mitf expression in the developing eye.
At stage 14, Mitf protein is detected throughout the RPE (Retinal Pigment Epithelium).

<Reference>
This antibody was used in the following references.

1. Tsukiji N et al “Mitf functions as an in ovo regulator for cell differentiation and proliferation during development of the chick RPE” Dev Biol 326: 335-346 (2009) PMID: 19100253
2. Delmas V et al “beta-Catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development” Genes Dev 21: 2923-2935 (2007) PMID: 18006687
3. Osawa M et al “Molecular characterization of melanocyte stem cells in their niche”Development 132: 5589-5599 (2005) PMID: 16314490

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