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marker of cancer stem cells in neurogenic tumors

Anti Nestin (ST1) polyclonal antibody

Background

Nestin is a class VI intermediate filament protein that is abundantly expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Upon differentiation, nestin becomes down-regulated and is replaced by other intermediate filament proteins. Nestin expression is widely used as a marker for CNS stem cells in the developing nervous system. Its transient expression is a critical step in the neural differentiation pathway. Down-regulated nestin may be re-expressed in the adult organism under certain pathological conditions such as brain injury, ischemia, inflammation, and neoplastic transformation.

Data Link : Swiss-Prot Q6P5H2 (mouse), P21263 (rat)

Application

1. Western blotting (dilution: 1/3,000-1/1,000)
2. Immunocytochemistry (dilution: 1/500-1/1,000)
3. Immunohistochemistry (dilution: 1/500-1/1,000)
    Not tested for other applications


Fig.1 Immunocytochemistry using this antibody, ST1 (ref.3).
BMP2 (bone morphogenetic protein 2)-induced neurogliogenic fate conversion of neural precursors.

Nestin expression (red) in neuroepithelial cells cultured with (B) or without (A) BMP2 (80 ng/ml) for 2 days was examined.
S100-β expression (green) was examined in neuroepithelial cells cultured with (D) or without (C) BMP2 (80 ng/ml) for 2 days.
Hoechst staining of the same fields (Insets, blue).

BMP2 dramatically decreased the number of nestin (a marker for undifferentiated neural precursor cells)-positive cells (B) compared with that in untreated cultures (A). The number of cells expressing S100- β (an astrocytic cell marker) was increased after 2 days of BMP2 stimulation (C and D). Thus BMP2 appears to change the fate of neural precursors from neuronal to astrocytic cells.

<Reference>
This antibody was produced and used in ref.2 and 3.

1. Lendahl U et al (1990) “CNS stem cells express a new class of intermediate filament protein.” Cell 60: 585-598 PMID: 1689217
2. Sato Y et al (1998) “Requirement for early-generated neurons recognized by monoclonal antibody Lot1 in the formation of lateral olfactory tract.” J Neurosci 18:7800-7810 PMID: 9742149
3. Nakashima K et al (2001) “BMP2-mediated alteration in the developmental pathway of fetal mouse brain cells from neurogenesis to astrocytogenesis.” Proc Natl Acad Sci USA 98: 5868-5873 PMID: 11331769

Product List

Product Name Cat# Quantity Price

Anti Nestin

BAM-73-105-EX 100UL

¥ 34,500
$ 460
€ 345

Anti MiTF

BAM-73-107-EX 100UL

¥ 34,500
$ 460
€ 345

Anti Nucleobindin 2/ NEFA / Nesfatin precursor

BAM-73-109-EX 100UL

¥ 34,500
$ 460
€ 345

Anti PDX1

KAL-KR059 25UG

¥ 49,000
$ 654
€ 490

Anti Initiate Factor 3 / IF3

KAL-KR070 25UG

¥ 49,000
$ 654
€ 490

Anti Keratin 12

KAL-KR074 25UG

¥ 49,000
$ 654
€ 490

Anti Hlxb9

KAL-KR077 25UG

¥ 49,000
$ 654
€ 490

Anti Calpain 6

KAL-KR084 50UG

¥ 49,000
$ 654
€ 490

Anti MFH

KAL-KJ091 50UG

¥ 55,000
$ 734
€ 550