The Alzheimer amyloid precursor protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APP ΔC31) and the 31-residues C-terminal fragment (APP-C31). APP ΔC31 potentially plays pathophysiological roles in neuronal death (ref.3). An antibody (named SAC) against the C-terminus of caspase 3-cleaved human APP695 (APP ΔC31) was raised in rabbit.
Data Link : Swiss-Prot P05067
1) Western blotting (dilution: 1/3,000-1/1,000)
2) Immunocytechemistry (dilution: 1/1,000-1/500)
Other applications have not been tested.
< Western blotting >
Human NT2 neurons (neurally differentiated human NT2 embryonic carcinoma cells) were infected with adenovirus vector expressing β-galactosidase (lane 1), wild-type APP (lane 2) or APPΔC31 (lane3). Cell lysates were prepared 48 h after infection, and proteins were analyzed by Western blotting using this antibody (SAC). Neurons overexpressing wild-type APP contained a 96 kD SAC-immunoreactive fragment which was also detected in APPΔC31-overexpressing neurons.
< Immunocytochemistry >
Caspase-3 activation and generation of the caspase-cleaved fragment APPΔC31 within neurons induced by serum deprivation (ref.3).
Neurally differentiated NT2 cells were cultured for 96 h in the absence of fetal calf serum. Cells were triply labeled for MAP2, the neuronal marker microtubule-associated protein 2 (MAP2), chromosomal DNA (Hoechst), and activated caspase-3 (ACP3; upper panel) or APPΔC31 (SAC; lower panel). MAP2-immunopositive neurons with apoptotic nuclei (arrows) are intensively immunostained with ACP3 and SAC.
This antibody was used in ref.3 and 4.
1. Kang HG et al. (1987) “The precursor of Alzheimer’s disease amyloid A4 protein resembles a cell-surface receptor.” Nature 325: 33-736 PMID: 2881207
2. Selkoe DJ (1994) “Normal and abnormal biology of the beta-amyloid precursor protein.” Annu. Rev. Neurosci. 17: 489-517 PMID: 8210185
3. Nishimura I et al. (2002) “Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein.” Cell Death Differ. 9: 199-208 PMID: 11840170
4. Nishimura I et al. (2003) “Upregulation and antiapoptotic role of endogenous Alzheimer amyloid precursor protein in dorsal root ganglion neurons.” Exp. Cell Res. 286: 241-251 PMID: 12749853
Anti APP (DELTA) C31