The Alzheimer amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation and neural plasticity. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates amyloid beta (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer’s disease patients. Isoform APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. An antibody (named AN2) against the -terminus of human APP (aa 18~38) was raised in rabbit.
Data Link: Swiss-Prot P05067
1) Western blotting (dilution: 1/3,000-1/1,000)
2) Immunocytechemistry (dilution: 1/1,000-1/500)
Other applications have not been tested.
< Western blotting >
Human NT2 neurons infected with adenovirus expressing β-galactosidase (lane 1) or wild-type APP (lane 2) were analyzed by Western blotting using this antibody. Wild-type APP was abundantly expressed in NT2 cells (ref.3).
Mouse dorsal root ganglion cells were treated with this antibody to examine neuronal APP expression (ref.4).
This antibody has been used in ref.3 and 4.
1. Kang HG et al. (1987) “The precursor of Alzheimer’s disease amyloid A4 protein resembles a cell-surface receptor.” Nature 325: 33-736 PMID: 2881207
2. Selkoe DJ (1994) “Normal and abnormal biology of the beta- amyloid precursor protein.” Annu. Rev. Neurosci. 17: 489-517 PMID: 8210185
3. Nishimura I et al. (2002) “Cell death induced by a caspase- cleaved transmembrane fragment of the Alzheimer amyloid precursor protein.” Cell Death Differ. 9: 199-208 PMID: 11840170
4. Nishimura I et al. (2003) “Upregulation and antiapoptotic role of endogenous Alzheimer amyloid precursor protein in dorsal root ganglion neurons.” Exp. Cell Res. 286: 241-251 PMID: 12749853