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Anti NADase (hemolytic streptococcus) polyclonal antibody

Background

NAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. Although the function as a toxin is largely unknown, it has been suggested to be related to pathogenicity of acute infection (1). NADase is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and the antibodies cross-react each other. Upon infection of hemolytic streptococci, the antibody titer to the NADase is increased similarly to anti-SLO (Strreptolysin O) antibody.
The product was prepared by immunizing rabbit with purified recombinant NADase of Group C hemolytic streptococci expressed in E, coli (2).

Application

1) Western blotting (x2,000~10,000 dilution)
2) Immunoprecipitation
3) Neutralization of NADase activity

BAM_64005_01.JPG
Detection of NADase in the culture supernatant of hemolytic streptococci
Lane 1; Culture supernatant of group A streptococcus
Lane 2; Culture supernatant of group C streptococcus
Lane 3; Culture medium only (negative control)

 

<Reference>
1.D.Stevens et al.:Molecular epidemiology of nga and NAD glycohydrolase/ADP-ribosyltransferase activity among Streptococcus pyogenes causing streptococcal toxic shock syndrome, J. Infect.Dis., 182, 1117-1128 (2000).
2.H.Kimoto et al.:Genetic and biochemical properties of streptococcal NAD-glycohydrolase inhibitor.J.Biol.Chem., 277, 14695-14702 (2006).

Product List

Product Name Cat# Quantity Price

Anti NADase

BAM-64-005-EX 50UL

¥ 30,000
$ 400
€ 300

Anti NADase

BAM-64-006-EX 250UL

¥ 90,000
$ 1200
€ 900