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Tag proteins antibody

Anti GFP Monoclonal Antibody (Clone No.1A5)

Background

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light (1). In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization (2, 3). The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Application

1) Western blotting (~1μg/ml)
2) Immuno-precipitation
3) Immunocytochemistry
4) Chromatin Immuno-Precipitation (ChIP)
5) ELISA

BAM_60001_01.jpg
Immunofluorescent staing of the COS1 cells with antibody 1A5

BAM_60001_02.jpg
GFP-expressed in COS1 cells

 

<Reference>
1. Shimomura O, Johnson F, Saiga Y (1962). "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea". J Cell Comp Physiol 59: 22339 PMID: 13911999
2. Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher D (1994). "Green fluorescent protein as a marker for gene expression". Science 263 (5148): 8025 PMID: 8303295 
3. Tsien R (1998). "The green fluorescent protein" (PDF). Annu Rev Biochem 67: 50944 PMID:97594961

Product List

Product Name Cat# Quantity Price

Anti GFP

BAM-60-001-EX 100UG

¥ 26,000
$ 347
€ 260