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Streptolysin O (Hemolytic streptococcus)

Background

Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin (2). SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci. The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo (Ref.1).

Application

1) Antigen for the measurement of anti-streptolysin 0 antibody (AS0) ( diagnostic reagent ).
2) Reagent for membrane pore formation to introduce small to macromolecules into living cells (Ref.1) or deplete intracellular ATP (Ref.3).

*This product is for research use only, not for human use. It should be handled carefully to avoid injection (mouse LD50, 8 ug/kg)

BAM_01501_01.jpg
SLO analyzed by SDS-PAGE.

 

<References>
This product has been used in Ref.3
1. Walev I. et al. Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O,PNAS, 98:3185 (2001)
2. M. Palmer: The family of thiol-activated, cholesterol-binding cytolysins. Toxicon, 39, 1681-1689 (2001).
3. Maeda Y. et al. GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. Nature Cell Biol. 10:1135-45(2008)

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

Bacterial Protein Toxins for Cell Biology and Biochemistry

  • Adenylate cyclase from Bordetella pertussis [BAM-01-501]
  • Pertussis toxin from Bordetella pertussis [BAM-01-503]
  • Dermonecrotizing toxin from Bordetella bronochiseptica [BAM-01-505]
  • Pasteurella multocida toxin [BAM-01-507]
  • Clostridium perfringens toxin [BAM-01-509]
  • Cholera toxin from Vibrio Cholerae [BAM-01-511]
  • Cholera toxin A subunit from Vibrio Cholerae [BAM-01-521]
  • Cholera toxin B subunit from Vibrio Cholerae [BAM-01-525]
  • Botulinum C3 enzyme from Clostridium botulinum [BAM-01-513]
  • Diphtheria toxin mutant CRM197 [BAM-01-515]
  • Diphtheria toxin [BAM-01-517]
  • Streptolysin O (Hemolytic streptococcus) [BAM-01-531]