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Anti Hepatitis C Virus (HCV) NS4a protein Monoclonal antibody

Background

Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A, non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9,400 bases which encodes a single polyprotein of about 3,010 amino acids (1, 2, 3) . The polyprotein is processed by host cell and viral proteases into four structural proteins (core, envelope1 and 2, and p7) and six non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viral replication. NS3 serine proteinase is responsible for proteolytic processing of other non-structural proteins. NS4a protein (54 amino acids) forms a comlex with NS3 and functions as a cofactor for NS3 protease activity.

Application

1. Western blotting
2. Immunofluorescence staining
3. ELISA

Other applications have not been tested.

BAM_65056_01.jpg
Fig.1  Western blotting of HCV NS4A protein.Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using anti-NS4a antibody. The protein detected with this antibody is 6 kD. This small NS4 protein (NS4a) was produced from the N-terminal region of the NS4 protein.

BAM_65056_02.jpg
Fig.2  Detection of HCV NS4a protein by immunofluorescence antibody staining.
Chimp liver cells were infected with recombinant
vaccinia virus containing a HCV genome cDNA.
After incubation for 48 hr, the cells were fixed with acetone and HCV NS4a protein was detected by indirect immunofluorescence staining using this antibody.

<Reference>
1. Brass V, Moradpour D, Blum HE. Molecular Virology of Hepatitis C Virus (HCV): 2006 Update. Int J Med Sci 2006; 3:29-34. PMID: 16614739
2. Kato,N. et al. (1990) “Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.” Proc. Natl. Acad. Sci. USA 87, 9524-9528 PMID: 2175903
3. Takamizawa, A. et al. (1991) “Structure and organization of the hepatitis C virus genome isolated from human carriers.” J.Virol.65, 1105-1113 PMID: 1847440  
4. Manabe,S. et al. (1994) “Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by N3.” Virology 198, 636-644 PMID: 8291245

Product List

Product Name Cat# Quantity Price

Anti HCV NS4a protein

BAM-65-057-EX 100UG

¥ 31,500
$ 420
€ 315

Anti HCV NS4a protein

BAM-65-058-EX 50UG

¥ 31,000
$ 414
€ 310

Anti HCV NS4a protein

BAM-65-059-EX 50UG

¥ 31,000
$ 414
€ 310

Anti HCV NS5a protein

BAM-65-062-EX 100UG

¥ 31,500
$ 420
€ 315

Anti HCV NS5a protein

BAM-65-063-EX 50UG

¥ 31,500
$ 420
€ 315

Anti HCV NS5a protein

BAM-65-064-EX 50UG

¥ 31,500
$ 420
€ 315

Anti HCV NS5b protein

BAM-65-067-EX 100UG

¥ 31,500
$ 420
€ 315

Anti HCV NS5b protein

BAM-65-068-EX 50UG

¥ 31,500
$ 420
€ 315

Anti HCV NS5b protein

BAM-65-069-EX 50UG

¥ 31,000
$ 414
€ 310

Anti HCV core (CP9)

SIM-2ZCP9 0.5ML

¥ 64,000
$ 854
€ 640

Anti HCV core (CP11)

SIM-2ZCP11 0.5ML

¥ 64,000
$ 854
€ 640

Anti HCV core (CP14)

SIM-2ZCP14 0.5ML

¥ 64,000
$ 854
€ 640