Antibodies for Proteoglycan Detection [PDF]
Versican, the product of the VCAN/CSPG2 gene, is a large extracellular matrix chondroitin sulfate proteoglycan ubiquitously expressed in interstitial matrices of the human body, including brain ECM [1-4]. It was first isolated from bovine aorta [5, 6] and later on isolated from the chick embryo . Cloning of the human VCAN/CSPG2 gene was accomplished in 1989 by D. Zimmermann and E. Ruoslahti , who also cognated the name versican in recognition of its versatile modular structure.
|Western blotting of purified human aorta versican (primarily a mixture of V1 and V2 isoforms) resolved
by SDS-PAGE on 3-8% linear gradient gels under reducing conditions
(MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase
ABC and endo-b-galactosidase pretreatment (Digested).
* Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the
intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore
agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic
deglycosylation, most isoforms still show complex, smeared banding patterns.
|Immunolocalization of versican isoforms recognized by mAb 6B10 in the indicated human
adult tissues (peroxidase reaction).
*mAb 6B10 detects specific versican V0-V2 isoforms widely distributed in connective tissues and particularly concentrated in vascular structures.
Chondroitinase ABC pre-digestion of the sections may affect the staining pattern.
Other species have not been tested.
Anti Versican (6B10)