1)Rat brain homogenates 7μg
The sample was prepared in the absence of phosphatase inhibitor.
2) Rat brain homogenates 7μg
The sample was prepared in the presence of phosphatase inhibitor
3) Rat brain homogenates 30μg
The sample was prepared in the absence of phosphatase inhibitor, and then it was phosphorylated by CaM kinase?
Preparation of antibodies and instruction:
Department of Molecular Pharmacology,
Kumamoto University Graduate shool of Medeical Science
|Package Size||25μg (100μL / vial)|
|Format||Rabbit polyclonal antibody 0.25mg/mL|
|Buffer||PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]|
|Storage||Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
|Purification method||This antibody was purified from rabbit serum immunized with partial peptide of human Tau Phospho-Specific (Ser416) by phosphorylated peptide affinity column.
And then, the antibody against unphosphorylated peptide was removed by unphosphorylated peptide affinity column.
|Working dilution||For western blotting ; 0.5〜2 μg/mL|
Anti Tau, phospho Ser416