The disruption of Spesp1 was shown to cause an aberrant distribution of various sperm proteins. SPESP1 is necessary to produce the fully ‘fusion competent’ sperm.
Fig.1 Analysis of PMIS2 protein expression in testes extracts from wild-type (+/+), and SPESP1single (+/-) and doble knockout mice by western blotting with anti-SPESP1 antibody. Testes and sperm were lyzed in lysis buffer containing 1% Triton-X100 and extracts were prepared as supernatants of lysates after centrifugation. Samples were reacted with anti-PMIS2 antibody at 1/1,000 dilution. The difference in predicted molecular mass (11 kDa) and the band position (18 kDa) of PMIS protein might be due to glycosylation.
Fig.2 Immunofluorescence staining of mouse SPESP1 with anti-SPESP1 antibody.
A. Round spermatid
B. Epididymal sperm
As secondary antibody, Alexa Fluor 488 conjugated anti-rabbit IgG antibody was used.
# Fujihara Y., et al. Sperm equatorial segment protein 1, SPESP1, is required for fully
fertile sperm in mouse. J Cell Sci. 2010 May 1;123(Pt 9):1531-6 PMID: 20375058
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.