This anti-rat macrophage/dendritic cells monoclonal antibody, RM-4, was produced by using a homogenate of silica-induced lung granulomas of rat as immunogen. Immunohistochemistry demonstrated that RM-4 is specific for rat macrophages and dendritic cell populations in various organs and tissues.
In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigen, of which molecular weight is 46kDa, presenting on the membrane of endosomes and lysosomes in macrophages and dendrictic cells.
Thus, this antibody is very useful for not only in discriminating macrophage/dendritic cells from various cell populations but also in identifying endosomes and lysosomes.
Rat liver (frozen section) Kupffer cells are positively staine
Rat spleen (frozen section)) Macrophages in red pulp and white pulp were positivery stained
|Takeya M., Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto, Japan|
|Spleen||Red pulp macrophages
IDCs in PALS
Marginal zone macrophages
|Lymph nodes||TBMs in follicles
IDCs in paracortical areas
|Thymus||Macrophages in cortex and medulla
IDCs in medulla
Twisted spindle shaped macrophages
|Peyer’s patches||Macrophages in the lymphoid follicle
Dendritic cells in the interfollicular area
|Brain||Microglial cells in the parenchyma
Macrophages in the subarachnoid spaces
Macrophages in the perivascular space
|Peritoneal cavity||Peritoneal macrophages|
TBM=tingible body macrophages; IDCs=interdigitating cells;
PALS=periarteriolar lymphatic sheath; LCs=Langerhans cells.
1. Iyonaga K., Takeya M., Yamamoto T., Ando M., Takahashi K. (1997) A novel monoclonal antibody, RM-4, specifically recognizes rat macrophages and dendritic cells in formalin-fixed, paraffin-embedded tissues. Histochem J. 29:105-116
|Package Size||50μg (200μL / viaL)|
|Format||Mouse monoclonal antibody 0.25mg/mL|
|Buffer||Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat|
|Storage||Store below 20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided
|Purification method||The spleen cells from BALB/c mouse, immunized with homogenate of silica-induced lung granuloma of rat, were fused to myeloma NS-1 cells. The screening of the hybridoma cells was performed on cryostat sections of rat spleens. The hybridoma cell line (RM-4) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.|
|Working dilution for immunohistochemistry: 10μg/mL on frozen sections and paraffin sections.|
Anti Macrophage / Dendritic Cells