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Anti Rad52 (human) polyclonal antibody

Background

Human Rad52 protein is a functional and structural homolog of yeast Rad52 protein, which plays a major role in genetic recombination and recombination repair by mediating strand annealing between homologous DNA strands (1). Rad51 functionally and physically interacts with Rad51 in recombination processes. It also interacts with oncogenes and tumor suppressors for the maintenance of genome stability (1).
The product was prepared by immunizing rabbit with full-size recombinant Rap52 protein expressed in E. coli and purified.
Using this anti-serum, Rad52 protein (46 kD) was detected by Western blotting (Fig Lane1 ) at 46 kD position .This antibody was successfully used for immunoprecipitatio assay (Fig. Lane2 ).

Application

1)  Western blotting (x 2,000)
2)  Immuno-precipitation
3)  Detection of foci formation by indirect immunofluorescence

Western blot and immuno-precipitation of Rad52
Lane1 : 1 ng / lane purified hRad52 protein
( products code BAM-10-003 )
Lane2 : immunoprecipitation sample (MCF7)

< Specification >
Reacts with Human and mammalian Rad51 protein

< Reference >
1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd ed., ASM Press