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useful for the research of the diabetic and Alzheimer's disease.

Anti Pyrraline Monoclonal Antibody

Background

Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE). Recent immunological studies using anti-AGE antibody (6D12) demonstrated the presence of AGE-modified proteins in several human tissues: (1) human lens (nondiabetic and noncataractous), (2) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (3) diabetic retina, (4) peripheral nerves of diabetic neuropathy, (5) atherosclerotic lesions of arterial walls, (6)β2-microglobulin forming amyloid fibrils in patients with hemodialysis-related amyloidosis, (7) senile plaques of patients with Alzheimer’s disease, (8) the peritoneum of CAPD patients, (9) skin elastin in actinic elastosis, and (10) ceriod/lipofuscin deposits. These results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease processes. This antibody named as 6D12 has been used to demonstrate AGE-modified proteins in these human tissues, indicating potential usefulness of this antibody for histochemical identification and biochemical quantification of AGE-modified proteins.

Pyrraline is one of the major Maillard compounds resulting from the reaction of glucose and amino coumpounds at slightly acidic pH. Using anti-pyrraline antibody, pyrraline was detected in sclerosed glomeruli from diabetic and normal old kidneys as well as in renal arteries with arteriosclerosis. Furthermore, it was detected in neurofibrillary tangles and senile plaques in brain tissue from patients with Alzheimer’s disease.

 AGEs Antibody Flyer [PDF]
 AGEs Antibody Flyer (print file) [PDF]
 
Package Size 20µg (80µL/vial)
Format
Buffer
Mouse monoclonal antibody 0.25 mg/mL
Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. H12
Subclass IgG1
Purification method The splenic lymphocytes from BALB/c mouse, immunized with pyrraline-HSA were fused to myeloma P3U1 cells. The cell line (H12) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: about 2µg/mL; for ELISA: 0.1-0.5µg/mL
References for Pyrraline
Miyata S,et al., J Clin Invest. 1992 Apr;89(4):1102-12. PMID: 1556177
Smith MA,et al.,Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5710-4. PMID: 8202552
C. Odetti P,et al.,Biochem Biophys Res Commun. 1998 Feb 24;243(3):849-51. PMID: 9501012

*These references are Pyrraline’s background, not examples of how Anti Pyrraline monoclonal antibody is used .
*This product was developed in conjunction with Meiji Milk Product Co.,LTD Institute of Health Science

References for AGEs antibody
Horiuchi S,et al., J Biol Chem. 1991 Apr 25;266(12):7329-32. PMID: 2019568
Araki N,et al., J Biol Chem. 1992 May 25;267(15):10211-4. PMID: 1587810
Miyata T,et al., J Clin Invest. 1993 Sep;92(3):1243-52 PMID: 8376584
Yamada K,et al., Clin Nephrol. 1994 Dec;42(6):354-61. PMID: 7882598
Kume S,et al., Am J Pathol. 1995 Sep;147(3):654-67. PMID: 7545874
Makino H,et al., Kidney Int. 1995 Aug;48(2):517-26. PMID: 7564121
Mori T,et al., Exp Mol Pathol. 1995 Oct;63(2):135-52. PMID: 8941048
Miyata T,et al., Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2353-8. PMID: 8637877
Kimura T,et al., Neurosci Lett. 1996 Apr 12;208(1):53-6. PMID: 8731173
Ikeda K,et al., Biochemistry. 1996 Jun 18;35(24):8075-83. PMID: 8672512

Product List

Product Name Cat# Quantity Price

Anti Pyrraline

KAL-KH010 20UG

¥ 55,000
$ 734
€ 550

Anti Pyrraline

KAL-KH010-01 20UG

¥ 70,000
$ 934
€ 700

Anti Pyrraline

KAL-KH010-02 20UG

¥ 70,000
$ 934
€ 700