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useful for immunohistochemical detection of POEM/nephronectin.

Anti POEM/nephronectin Polyclonal Antibody

Background

POEM (Pre osteoblastic EGF repeat protein with MAM domain)/nephronectin is a novel ligand for α8β1 integrin. Its mRNA and protein is widely expressed in developing embryo, such as kidney, bone, muscles and endocrine organs except uriniferous tubule after childbirth. POEM/nephronectin estimated to encode a 60k dalton protein which has several functional domains, such as a signal sequence, five EGF-like repeated sequences, a proline-rich domain, a RGD cell binding motif and a MAM domain. POEM/nephronectin protein is firmly bound to the cell surface after its secretion and not observed in culture medium. Recent research results indicate that not only the MAM domain but also the RGD cell binding motif in POEM/nephronectin protein plays an important role in cell adhesion, spreading, and survival.
This antibody was established from the purified serum immunized with a short peptide of mouse POEM/nephronectin. It is useful for immunohistochemical detection of POEM/nephronectin.

 

Package Size 50 μg (200 μL/vial)
Format
Buffer
Rabbit polyclonal antibody 0.25mg/mL
PBS [containing 50% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Storage
Purification method
Below -20°C
This antibody was purified from rabbit serum by affinity chromatography.
Working dilution For Immunohistochemistry ; 0.2〜0.5 μg/mL

 

Immunohistochemistry

Sample : Mouse Kidney
left; Anti POEM/nephronectin Antibody
right; control (Rabbit IgG)

Preparation of antibodies and instruction :
Drs. Tezuka K. at Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

“Anti POEM/nephronectin Polyclonal Antibody” also belongs to the following categories.