PMIS2 might function both as the ultimate factor regulating sperm transport into the oviducts and in modulating sperm–zona binding.
Fig.1. Analysis of PMIS2 protein expression in testes extracts from wild-type (+/+) and Pmis2 knockout (-/-) mice by western blotting with anti-PMIS2 antibody. Testes and sperm were lyzed in lysis buffer containing 1% Triton-X100 and extracts were prepared as supernatants of lysates after centrifugation. Samples were reacted with anti-PMIS2 antibody at 1/1,000 dilution. The difference in predicted molecular mass (11 kDa) and the band position (18 kDa) of PMIS protein might be due to glycosylation.
# Yamaguchi R., et al. Mice expressing aberrant sperm-specific protein PMIS2 produce
normal-looking but fertilization-incompetent spermatozoa. Mol Biol Cell. 2012
Jul;23(14):2671-9. PMID: 22621904
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.