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useful for the research of the diabetic, kidney failure, arterial sclerosis,Alzheimer's disease, rheumatoid arthritis and atopic dermatitis

Anti Pentosidine Monoclonal Antibody

Background

Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recent immunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteins in several human tissues: (ⅰ) human lens (nondiabetic and noncataractous), (ⅱ) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (ⅲ) diabetic retina, (ⅳ) peripheral nerves of diabetic neuropathy, ( ⅴ ) atherosclerotic lesions of arterial walls, ( ⅵ ) β 2-microglobulin forming amyloid fibrils in patients with hemodialysis-related amyloidosis, (ⅶ) senile plaques of patients with Alzheimer's disease, (ⅷ) the peritoneum of CAPD patients, (ⅸ) skin elastin in actinic elastosis, and (ⅹ) ceriod / lipofuscin deposits. These results suggest a potential role of AGEs-modification in normal aging as well as age-enhanced disease processes. This antibody named as 6D12 has been used to demonstrate AGEs-modified proteins in these human tissues, indicating potential usefulness of this antibody for histochemical identification and biochemical quantification of AGEs-modified proteins. Pentosidine is one of the Maillard compounds identified by Monnier et al in 1989. It has been proved to cross-link Arginine to Lysine residue and be detected in β 2-microglobulin from patients with hemodialysis- related amyloidosis.

Pentosidine

 AGEs Antibody Flyer [PDF]
 AGEs Antibody Flyer (print file) [PDF]
 
Package Size 50µg (200µL/vial)
Format
Buffer
Mouse monoclonal antibody 0.25 mg/mL
Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. PEN-12
Subclass IgG1
Purification method The splenic lymphocytes from BALB/c mouse, immunized with pentosidine-HSA were fused to myeloma P3U1 cells. The cell line (PEN-12) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 5-10µg/mL; for ELISA: 0.1-1.0µg/mL
Specificity Reaction to pentosidine-HSA was suppressed by free-pentosidine in competitive ELISA.
References
Sell DR,et al., J Biol Chem. 1989 Dec 25;264(36):21597-602. PMID: 2513322
Miyata T,et al., Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2353-8. PMID: 8637877

*These references are the background of Pentosidine , and are not this antibody examples .

Product List

Product Name Cat# Quantity Price

Anti Pentosidine

KAL-KH012 50UG

¥ 55,000
$ 734
€ 550

Anti Pentosidine

KAL-KH012-01 50UG

¥ 70,000
$ 934
€ 700

Anti Pentosidine

KAL-KH012-02 50UG

¥ 70,000
$ 934
€ 700

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.
This product was developed in conjunction with Meiji Milk Product Co.,LTD Institute of Health Science.
This product cannot be sold to USA because of the patent issue.