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Anti Osteopontin (OPN)

Background

Osteopontin (Early T-Lymphocyte Activation-1: Eta-1) is a highly phosphorylated and glycosylated phosphoprotein that is expressed in many tissues and has a variety of function, cell adhesion, inhibition of calcium oxalate crystal growth and intracellular signalling. . Osteopontin(OPN) has a cell surface-binding sequence (RGDS), a thrombin cleavage site, many asparatic acid residues, and a number of phosphorylation site. We provide you native form OPN, the monoclonal antibodies and ELISA kit. Human Milk Osteopontin(OPN) is purified from the human first milk by two-step purification procedure as an intact form (50kDa - 80kDa). Anti-OPN monoclonal antibodies were prepared with this native form OPN.

Feature and Advantages

Product : Anti-OPN Monoclonal Antibody
Features : Prepared with native OPN
There are several kinds of clones (See Epitope Analysis)
Use : Detection of native OPN or this fragments with ELISA, western blotting analysis.

Application Examples

Western Blotting analysis with monoclonal antibody from hybridoma clone 8H4. Western Blotting with clone 5D10 Western Blotting with clone 8E5

  Peptide
Clone Name GST/OPN-a GST/OPN-b GST/OPN-c GST/OPN-d GST/OPN-e Purified human milk OPN
2B4 O O O O x O
5D10
(IgG1 K)
O O O O x O
6C3
(note)
O O O x x O
6E5 O O O x O O
7A8 x x x x x O
8B12 O
8E5
(IgG1 K)
O O x O x O
8H4 O x x x x O
10G7 x x x x x O
12D4 O O O O x O
17C5 O O x O x O

O : reacts, △ : reacts weakly, x : none react, note : 6C3 recognized Thrombin Cleavage Site.

Product List

Product Name Cat# Quantity Price

Anti Osteopontin

LSL-LB-4225 100UL

¥ 50,000
$ 667
€ 500

References
  • Tartrate-Resistant Acid Phosphatase Knockout Mice.
    Hayman AR, Cox TM. J Bone Miner Res. 2003 Oct;18(10):1905-7.PMID: 14584904
  • TRACP as an osteopontin phosphatase.
    Andersson G, Ek-Rylander B, Hollberg K, Ljusberg-Sjölander J, Lång P, Norgård M, Wang Y, Zhang SJ.
    J Bone Miner Res. 2003 Oct;18(10):1912-5. PMID: 14584906
  • Purification and characterization of osteopontin from human milk.
    Sørensen S, Justesen SJ, Johnsen AH.
    Protein Expr Purif. 2003 Aug;30(2):238-45. PMID: 14584906
  • Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles.
    Sasano Y, Zhu JX, Kamakura S, Kusunoki S, Mizoguchi I, Kagayama M.
    Anat Embryol (Berl). 2000 Jul;202(1):31-7. PMID: 10926093
  • Bone matrix proteins in osteogenesis and remodelling in the neonatal rat mandible as studied by immunolocalization of osteopontin, bone sialoprotein, alpha 2HS-glycoprotein and alkaline phosphatase.
    Pinero GJ, Farach-Carson MC, Devoll RE, Aubin JE, Brunn JC, Butler WT.
    Protein Expr Purif. 2003 Aug;30(2):238-45. PMID: 7794128
  • Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.
    Rittling SR, Matsumoto HN, McKee MD, Nanci A, An XR, Novick KE, Kowalski AJ, Noda M, Denhardt DT.
    Protein Expr Purif. 2003 Aug;30(2):238-45. PMID: 9661074
  • Localization of transglutaminase-reactive glutamine residues in bovine osteopontin.
    Sørensen ES, Rasmussen LK, Møller L, Jensen PH, Højrup P, Petersen TE.
    Biochem J. 1994 Nov 15;304 ( Pt 1):13-6. PMID: 7998923
  • Termine, J.D. and Robey, P.G.: Bone matrix proteins and the mineralization process. In: Primer on the metabolic bone diseases and disorders of mineral metabolism, ed. by Favus, M.J., Lippincott-Raven, Philadelphia, 1996, PP.24-28.