The main constituents of biological membrane are the glycerophospholipids. Besides variety in their hydrophilic portions, phospholipids comprise various molecular species with different fatty acid moieties. Among those varieties,this antibody selectively binds to the phosphatidylcholine with oleic acid at the sn-1 site. This type of lipid is concentrated at the tip of neuronal protrusions.
|Immno-electron microscopy||3 μg/ml ( Ref.1, Fig. 1 )|
|Fluorescence - activated cell sorter||1 μg/ml ( Ref. 1, Fig. 3 )|
|Immuno-fluorescence cell staining||0.3 μg/ml ( Ref.1, Fig. 1 )|
|ELISA||3 μg/ml ( Ref.1, Fig. 2 )|
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
OPPC localization analysis on cultured nerve cell (rat PC12 cell, clone#15-3C1)
|Immunogen||PC12 raft fraction|
|Rased in||Mouse (Balb/c)|
|Clone number||15 - 3C1|
|Source||Culture supernatant (+10%FBS)|
|Buffer||RPMI + 0.025% NaN3 as a preservative|
|Concentration||90 μg / ml|
|Antigen||PC12 raft fraction|
Anti 1-Oleoyl-Palmitoyl-Phosphatidylcholine (OPPC)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.