a1-antitrypsin(AAT) is one of the serine proteinase inhibitors that are present in human serum. In sera, AAT is present as native AAT, which inhibits serine proteinase and elastase-AAT complex and oxidized AAT(ox-AAT), which is oxidized by reactive oxygen species from activated neutrophils, resulting in a loss of activity.
It is considered that ox-AAT is a marker of oxidative stress and is related to pulmonary enphysema or COPD
The antibody (3F4) recognizes the ox-AAT, and S3-1 recognizes the native AAT.
These antibodies can be used for EIA, western blotting, immunohistochemical staining, and using this antibody, ox-AAT can be distinguished from native AAT.
1. Ueda M, Mashiba S, Uchida K. Evaluation of oxidized alpha-1-antitrypsin in blood as an oxidativestress marker using anti-oxidative a1-AT monoclonal antibody. Clinica Chimica Acta 2002; 317: 125-131.
2. Mashiba S, Wada Y, Takeya M, Sugiyama A, Hamakubo T, Nakamura A, Noguchi N, Niki E, Izumi A, Kobayashi M, Uchida K, Kodama T. In vivo complex formation of oxidized a1-antitrypsin and LDL. Arterioscler Thromb Vasc Biol 2001; 21: 1801-1808.
Anti Native ALPHA1 Antitrypsin
Anti Oxidized ALPHA1 Antitrypsin
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.