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Anti Myristoylated alanine-rich C kinase substrate [MARCKS] Monoclonal Antibody

For Research Use Only

Background

Myristoylated alanine-rich C kinase substrate (MARCKS), a specific substrate for protein kinase C, is abundant in the brain. MARCKS binds calmodulin under Ca2+  or actin under non-Ca2+ conditions. Phosphorylation of MARCKS (P-MARCKS) can be used as an indicator of protein kinase C activation in intact cells. It has been shown that P-MARCKS has relatively weaker binding activity to calmodulin and actin compared with MARCKS.
Pathophysiologically, P-MARCS is also detected in the brain of Alzheimer’s disease patients and in the smooth muscle cells of arteriosclerosis.

This antibody could be used for western blotting and immunohistchemistry.

Package Size 50μg  (200μL/vial)
Format
Buffer
Rat monoclonal antibody 0.25 mg/mL
Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below 20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. MAR11/2
Subclass IgG2a
Purification method The lymphocytes from rat, immunized with C terminal peptides of rat MARCKS, were fused to mouse myeloma SP2 cells. The cell line (MAR11/2) with positive reaction was grown in serum free medium from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: about 1~ 5μg/mL

The reactivity of each antibody to MARCKS and P-MARCKSusing western blotting.

Rane No.
1:  C6 cell activated by beta-PMA 0 min
2:  C6 cell activated by beta-PMA 15 min
3:  C6 cell activated by beta-PMA 30 min
4:  C6 cell activated by beta-PMA 45 min
5:  C6 cell activated by beta-PMA 60 min
6:  C6 cell no treatment (DMSO)

Product List

Product Name Cat# Quantity Price

Anti MARCKS

KAL-KT042 50UG

¥ 55,000
$ 734
€ 550