For Research Use Only
Myristoylated alanine-rich C kinase substrate (MARCKS), a specific substrate for protein kinase C, is abundant in the brain. MARCKS binds calmodulin under Ca2+ or actin under non-Ca2+ conditions. Phosphorylation of MARCKS (P-MARCKS) can be used as an indicator of protein kinase C activation in intact cells. It has been shown that P-MARCKS has relatively weaker binding activity to calmodulin and actin compared with MARCKS.
Pathophysiologically, P-MARCS is also detected in the brain of Alzheimer’s disease patients and in the smooth muscle cells of arteriosclerosis.
This antibody could be used for western blotting and immunohistchemistry.
|Package Size||50μg (200μL/vial)|
|Rat monoclonal antibody 0.25 mg/mL
Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
|Storage||Store below 20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
|Purification method||The lymphocytes from rat, immunized with C terminal peptides of rat MARCKS, were fused to mouse myeloma SP2 cells. The cell line (MAR11/2) with positive reaction was grown in serum free medium from which the antibody was purified by Protein G affinity chromatography.|
|Working dilution for immunohistochemistry: about 1~ 5μg/mL|
The reactivity of each antibody to MARCKS and P-MARCKSusing western blotting.
1: C6 cell activated by beta-PMA 0 min
2: C6 cell activated by beta-PMA 15 min
3: C6 cell activated by beta-PMA 30 min
4: C6 cell activated by beta-PMA 45 min
5: C6 cell activated by beta-PMA 60 min
6: C6 cell no treatment (DMSO)