TRP (transient receptor potential) channels comprise a superfamily of non-selective cation channels with at least nine subfamilies. The variety of subfamilies corresponds to the differences in the activation mechanisms and functions.
TRPV4 (TRP vanilloid 4), first identified as an osmosensory ion channel, can also be activated by warm temperatures (> 27-35 degrees C). TRPV4 is expressed in a wide variety of tissues (sensory neurons, hypothalamus, skin, kidney, lung, inner ear). TRPV4 is a primary afferent transducer that plays a crucial role in neuropathic hyperalgesia for osmotic and mechanical stimuli, as well as in inflammatory mediator-induced hyperalgesia for osmotic stimuli. It functions as a Ca 2+ entry channel and can be activated by a wide range of stimuli including physical (cell swelling, heat, mechanical stimulation) and chemical stimuli (endocannabinoids, arachidonic acid metabolites, and 4alpha-phorbol esters). Moreover, TRPV4 plays a major role in mechanical hyperalgesia and enhanced nociception to hypo-asmotic stimuli by Taxol.
Given its wide expression and the variety of activatory stimuli, TRPV4 is likely to play a number of physiological roles. Studies with TRPV4(-/-) mice suggest a role for the channel in the regulation of body osmolarity, mechanosensation, and temperature sensing.
This antibody will be very useful to research osmotic, mechanical stimuli, inflammatory reaction and thermosensitive response
|Package Size||25g (100L/vial)|
|Format||Rabbit polyclonal antibody 0.25mg/mL|
|Buffer||PBS [containing 2% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]|
|Storage||Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
|Purification method||This antibody was established from the serum of a rabbit immunized with the partial peptide corresponding to amino acid 3-17 of mouse TRPV4, and purified by peptide affinity chromatography.|
|Working dilution||For Western blotting: 0.25 g/ml|
Lane 1: choroid plexus (Wild type mouse)
Lane 2: choroid plexus (TRPV4 knockout mouse)
Lane 3: rat TRPV4 overexpressed in HEK293 cells
Preparation of antibodies and instruction
Dr. Makoto Tominaga at Section of Cell Signaling,
Okazaki Institute for Integrative Bioscience,
National Institutes of Natural Sciences
Anti TRPV 4
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.