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useful to research the nocifensive (thermosensitive and mechanosensitive) response to pain.

Anti Mouse TRPA1 Polyclonal Antibody

Background

Mammals feel a wide range of temperature spanning from cold to heat with specialized neurons in the peripheral nervous system. With this range, temperatures over about 43 degrees C and below about 15 degrees C evoke not only a thermal sensation, but also a feeling of pain.
Nine thermosensitive ion channels have been reported, all of which belong to TRP (transient receptor potential) superfamily. They are expressed in sensory tissues, such as nociceptors and skin. Among them, TRPA1 (TRP cation channel, subfamily A, member 1) has been identified as cold-sensitive ion channel. TRPA1 is activated at approximately 17 degrees C, a temperature that is reported as painfully cold by humans.
In addition to noxious cold, TRPA1 is activated pungent ingredients present in mustard, garlic, ginger, clove, wintergreen and cinnamon all and found in a subset of nociceptive sensory neurons where it is coexpressed with TRPV1/VR1 (capsaicin/heat receptor). Moreover, TRPA1 has been proposed to be a component of the mechanosensitive transduction channel of vertebrate hair cells.

 

This antibody will be very useful to research the nocifensive (thermosensitive and mechanosensitive) response to pain.

 

Package Size 50g(200L/vial)
Format Rabbit polyclonal antibody  0.25mg/mL
Buffer PBS [containing 2% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of a rabbit immunized with the partial peptide corresponding to amino acid 12-24 of mouse TRPA1, and purified by peptide affinity chromatography.
Working dilution For Western blotting :1.6 g/ml
For Immunoprecipitation :1.6 g/ml

 

Sample: HEK293 cells overexpressing mouse TRPA1 (cell membrane fraction)

Left: Western blotting
Right: Immunoprecipitation

Preparation of antibodies and instruction
Dr. Makoto Tominaga at Section of Cell Signaling,
Okazaki Institute for Integrative Bioscience,
National Institutes of Natural Sciences

Product List

Product Name Cat# Quantity Price

Anti TRPA 1

KAL-KM120 50UG

¥ 49,000
$ 654
€ 490

Background

Mammals feel a wide range of temperature spanning from cold to heat with specialized neurons in the peripheral nervous system. With this range, temperatures over about 43 degrees C and below about 15 degrees C evoke not only a thermal sensation, but also a feeling of pain.
Nine thermosensitive ion channels have been reported, all of which belong to TRP (transient receptor potential) superfamily. They are expressed in sensory tissues, such as nociceptors and skin. Among them, TRPA1 (TRP cation channel, subfamily A, member 1) has been identified as cold-sensitive ion channel. TRPA1 is activated at approximately 17 degrees C, a temperature that is reported as painfully cold by humans.
In addition to noxious cold, TRPA1 is activated pungent ingredients present in mustard, garlic, ginger, clove, wintergreen and cinnamon all and found in a subset of nociceptive sensory neurons where it is coexpressed with TRPV1/VR1 (capsaicin/heat receptor). Moreover, TRPA1 has been proposed to be a component of the mechanosensitive transduction channel of vertebrate hair cells.

 

This antibody will be very useful to research the nocifensive (thermosensitive and mechanosensitive) response to pain.

 

Package Size 50g(200L/vial)
Format Rabbit polyclonal antibody  0.25mg/mL
Buffer PBS [containing 2% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of a rabbit immunized with the partial peptide corresponding to amino acid 12-24 of mouse TRPA1, and purified by peptide affinity chromatography.
Working dilution For Western blotting :1.6 g/ml
For Immunoprecipitation :1.6 g/ml

 

Sample: HEK293 cells overexpressing mouse TRPA1 (cell membrane fraction)

Left: Western blotting
Right: Immunoprecipitation

Preparation of antibodies and instruction
Dr. Makoto Tominaga at Section of Cell Signaling,
Okazaki Institute for Integrative Bioscience,
National Institutes of Natural Sciences