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Stem cell marker Anti Mouse Nanog Antibody

Background

Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified homeodomain-bearing transcriptional factor. Importantly, Nanog is expressed specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.

Application

Western blot : Use at a dilution of 1/300 - 1/2000
Immunocytochemistry: Use at a dilution of 1/150 - 1/700

<Reference>
1. Mitsui, K. et al. : Cell. 2003 May 30;113(5):631-642.
2. Hatano, SY. et al. : Mech Dev. 2005 Jan;122(1):67-79.
3. Yamaguchi, S., Kimura, H., Tada, M., Nakatsuji, N. and Tada, T. (2005) Nanog expression in mouse germ cell development. Gene Expr Patterns 5, 639-646.

Character

Host Rabbit
Immunogen Mouse Nanog protein
Clonality Polyclonal
Purity Immunogen affinity purified
Concentration 0.2 mg/mL
Storage Buffer PBS(incl.0.1% sodium azide)
Storage 4 °C (Short term)
-20 or -80 °C (Long term)

Product List

Product Name Cat# Quantity Price

Anti Nanog

REC-RCAB001P 200UL

¥ 54,000
$ 720
€ 540

Anti Nanog

REC-RCAB002P-F 100UL

¥ 28,500
$ 380
€ 285