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Anti mouse CD163 [Cluster of Differentiation 163]

Background

CD163 is a scavenger receptor for the hemoglobin-haptoglobin complex. It has also been shown to mark cells of monocyte/macrophage lineage. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants.

Application

Application notes & Recommended dilutions

Flow cytometry: 1/200

IHC: 1/1000

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Fig.1 Flow cytometry: Untransfected (red) and transfected with ICAFectinR 441 and plasmid encoding mouse CD163 Hela cells were stained with rabbit anti-mouse CD163 polyclonal antibodies diluted 1/100 (orange) and 1/200 (blue) and R-PE goat anti rabbit IgG secondary antibody at the dilution 1/200.

Fig.2 Immunohistochemistry: Hela cells transfected with ICAFectinR 441 and plasmid encoding mouse CD163 were fixed 24h post transfection, permeabilized and stained with rabbit anti-mouse CD163 Polyclonal Antibodies diluted 1/20 and Alexa488 goat anti rabbit IgG secondary antibody at dilution 1/1000. Nuclei were counterstained with DAPI in blue.

Product List

Product Name Cat# Quantity Price

Anti CD163

CAC-ICA-TG4-MSP1 50UL

¥ 60,000
$ 800
€ 600

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.