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Anti LC3 (Clone: LC3.No.6) monoclonal antibody

Background

LC3B is one of the mammalian Atg8 homologs and widely used as an autophagosome marker. Immediately after synthesis, LC3 is processed by Atg4 and becomes LC3-I. Upon induction of autophagy, the C-terminal glycine of LC3-I is conjugated to phosphatidylethanolamine, resulting in formation of membrane-bound LC3-II. Most LC3-II is thought be present on autophagosome membrane. The autophagosome subsequently fuses with a lysosome, where inside materials, including LC3-II, are degraded. The expression level of LC3-II generally correlates with the number of autophagosome

Application

Western Blot: 1/500-1/1,000.
Predicted molecular weight: LC3-I is 18 kDa and LC3-II is 16 kDa

MEF +/- Starvation 2 hr
SDS-PAGE (MEF lysate 15 µ/lane, 15% gel)
Transfer to PVDF membrane (semi-dry blotter, 100 mA/gel 1 hr)
Immunoblot for LC3B
Expose ECL 5min

Optimum dilution rate should be determined by end users

Applying Campaign Price (from July 1st - November 30th, 2017)

⇒ Autophagy antibody products 30% discount campaign

Product List

Product Name Cat# Quantity Price

Anti LC3

CAC-CTB-LC3-1-50 50UG

¥ 35,000
$ 667
€ 500

Citation
  • Tenovin-6 impairs autophagy by inhibiting autophagic flux.
    Yuan H, Tan B, Gao SJ.
    Cell Death Dis. 2017 Feb 9;8(2):e2608. PMID: 28182004
  • Accumulation of p62 in degenerated spinal cord under chronic mechanical compression: functional analysis of p62 and autophagy in hypoxic neuronal cells.
    Tanabe F, Yone K, Kawabata N, Sakakima H, Matsuda F, Ishidou Y, Maeda S, Abematsu M, Komiya S, Setoguchi T.
    Autophagy. 2011 Dec;7(12):1462-71. PMID: 22082874
  • Autophagy impairment stimulates PS1 expression and gamma-secretase activity.
    Ohta K, Mizuno A, Ueda M, Li S, Suzuki Y, Hida Y, Hayakawa-Yano Y, Itoh M, Ohta E, Kobori M, Nakagawa T.
    Autophagy. 2010 Apr;6(3):345-52. PMID: 20168091

References
  • How to interpret LC3 immunoblotting.
    Mizushima N, Yoshimori T.
    Autophagy. 2007 Nov-Dec;3(6):542-5. PMID: 17611390
  • LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
    Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T.
    EMBO J. 2000 Nov 1;19(21):5720-8. PMID: 11060023

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.