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Anti LC3 (Clone: LC3.No.6) monoclonal antibody


LC3B is one of the mammalian Atg8 homologs and widely used as an autophagosome marker. Immediately after synthesis, LC3 is processed by Atg4 and becomes LC3-I. Upon induction of autophagy, the C-terminal glycine of LC3-I is conjugated to phosphatidylethanolamine, resulting in formation of membrane-bound LC3-II. Most LC3-II is thought be present on autophagosome membrane. The autophagosome subsequently fuses with a lysosome, where inside materials, including LC3-II, are degraded. The expression level of LC3-II generally correlates with the number of autophagosome


Western Blot: 1/500-1/1,000.
Predicted molecular weight: LC3-I is 18 kDa and LC3-II is 16 kDa

MEF +/- Starvation 2 hr
SDS-PAGE (MEF lysate 15 µ/lane, 15% gel)
Transfer to PVDF membrane (semi-dry blotter, 100 mA/gel 1 hr)
Immunoblot for LC3B
Expose ECL 5min

Optimum dilution rate should be determined by end users

Product List

Product Name Cat# Quantity Price

Anti LC3

CAC-CTB-LC3-1-50 50UG

¥ 50,000
$ 667
€ 500

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    Cell Death Dis. 2017 Feb 9;8(2):e2608. PMID: 28182004
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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.