PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

Antibody to confirm stem cell quality

Anti KS [Keratan Sulfate] (R-10G)

Background

Monoclonal antibody R-10G recognises Keratan Sulfate lacking oversulfated structures in oligosaccharide segments of Keratan Sulfate glycosaminoglycan chains on hiPS cells. Digestion with Keratanase II, Keratanase or Endo-β-galactosidase removes these epitopes from Keratan Sulfate proteoglycans. R-10G is useful as a potent tool for the evaluation and standardization of hiPS cells in regenerative medicine.

Pluripotent cell epitope on the polycadixin molecular structure

Applications

Fig.1 Screening of R-10G mAb by western blotting.
Tic cell lysates in the complete RIPA buffer were desolved by SDS-PAGE on a 4-15% gradient gel under nonreducing, followed by immunoblot detection with R-10G. A : GelCode* Blue staining of SDS-PAGE of the Tic cell lysates (10ug protein). B : Tic cell lysates were analyzed by western blotting with R-10G. The molecular mass markers are shown on the left.


Fig. 2. Localization of the R-10G and TRA-1-81 epitopes on cultured Tic cells visualized on laser confocal microscopy.
Tic cells cultured on Millicell* EZ slides were double-stained first with R-10G and Alexa Fluor 488-conjugated secondary (anti-mouse IgG1) antibody, followed by with TRA-1-81 and Alexa Fluor 555-conjugated secondary (anti-mouse IgM) antibody. Cells were observed at two different magnifications: ?~100 (upper panel) and ?~400 (lower panel). (I) Nomarski imaging. (II) Nuclear counterstaining with TO-PRO*-3. (III) Antigens for R-10G (green). (IV) Antigens for TRA-1-81 (conventional hiPS marker antibody) (red). (V) Merged image of (III) and (IV). (VI) Close-up view of V (?~400).

Product List

Product Name Cat# Quantity Price

Anti Keratan Sulfate (R-10G)

CAC-RIT-M001 100UG

¥ 40,000
$ 534
€ 400

References
  •  Kawabe K.,Kawabe K et al., A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures. Glycobiology. 2013 Mar;23(3):322-36. doi: 10.1093/glycob/cws159. Epub 2012 Nov 15. PMID: 23154990

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.