IGSF8 may play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
Fig 1. Analysis of IGSF8 protein in various tissues of Igff8-targeted mice by western blotting with anti-IGFS8 antibody. Lysates of tissues (30 µg) were analyzed by western blotting using the antibody at 1/500 dilution. ?g F ?h and ?g - ?g stand for floxed and knock-out alleles, respectively.
Fig.2. Immunofluorescence staining of IGSF8 protein in eggs of wild-type mouse and Igsf8 knock-out mouse with anti-IGSF8 antibody. Zona-free eggs were fixed in PBS containing 0.5% (v/v) polyvinylpyrrolidone and 4% (v/v) paraformaldehyde. The anti-IGSF8 antibody was used at 1/100 dilution and as the second antibody, Alexa-Fuor 546 labeled anti-rabbit IgG was used (red). Then the DNA was stained with Hoechst 33342 (blue). ?gDIC?h is?@picture of Differential Interferernce Contrast microscopy.
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