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Useful for the research of macrophage that permeates through organization and analysis of various conditions

Anti Human Macrophage Surface Antigen Monoclonal Antibody

Back to Macrophage Antibodies

Background

This anti-macrophage monoclonal antibody, AM-3K, was produced by using human alveolar macrophages as immunogen. AM-3K reacts intensely with most of macrophages in lymphoreticular organs and in many other organs and tissues. AM-3K also reacts with the macrophages in many pathological conditions. However, this antibody does not react with dendritic cell population, such as epidermal Langerhans cells, interdigitating cells in the paracortex of lymph nodes, nor follicular dendritic cells. Lymphocytes, granulocytes and freshly isolated blood monocytes are also negative. Reaction products for AM-3K were found on the cytoplasmic membrane of macrophages by immunoelectron microscopy.
In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigen presenting on the cell surface membrane of tissue macrophages, but not monocytes or dendritic cells.
The molecular weights of the antigen recognized by AM-3K are 120 and 70 kDa.


Human liver (paraffin section)
Kupffer cells are positivery stained

Human lymph node (paraffin section)
Macrophages in marginal sinus are positivery stained.
Takeya M., Second Department of Pathology, Kumamoto
University School of Medicine, Kumamoto, Japan

Specificity

Organ reaction
positive negative
Thymus Macrophages in cortex
Macrophages in medulla
 
Spleen Red pulp macrophages
White pulp marginal zone macrophages
IDCs in PALS
Lymph nodes TB macrophages in follicles
Sinus macrophages
IDCs in paracortical areas
Lungs Alveolar macrophages  
Liver Kupffer cells  
Skin Dermal macrophages Langerhans cells
Brain Microglial cells  
Others   Renal tubules
Blood monocytes

PALS=periarteriolar lymphatic sheath; TB=tingible body; IDCs=interdigitating cells

Interspecies reactivity

Positive: Human, Monkey, Horse, Bovine, Pig, Goat, Dog, Cat, Rabbit, Guinea pig

Package Size 50μg (200μL / vial)
Format Mouse monoclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below 20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided
Clone No. AM-3K
Subclass IgG1
Purification method The spleen cells from BALB/c mouse, immunized with human alveolar macrophages, were fused to myeloma NS-1 cells. The screening of the hybridoma cells was performed on cryostat sections of human lung. The cell line (AM-3K) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 10μg/mL, on frozen sections and paraffin sections.
Antigen retrieval (microwave 10min, 0.01M citrate buffer, pH2.0) recommended.

 

 

<Reference>

1. Zeng L., Takeya M., and Takahashi K. (1996) AM-3K, A novel monoclonal antibody specific for tissue macrophages and its applications to pathological investigation. Journal of Pathology 178 : 207-214

2. Yamate J., Yoshida H., Tsukamoto Y., Ide M., Kuwamura M., Ohashi F., Miyamoto T., Kotani T., Sakuma S., Takeya M. (2000) Distribution of cells immunopositive for AM-3K, a novel monoclonal antibody recognizing human macrophages, in normal and diseased tissues of dogs, cats, horses, cattle, pigs and rabbits. Vet Pathol 37(2): 168-176

3. Zeng L., Takeya M., Ling X., Nagasaki A., Takahashi K. (1996) Interspecies reactivities of anti-human macropharge monoclonal antibodies to various animal species. J Histochem Cytochem 44(8): 845-853

4. Frangogiannis NG., Burns AR., Micheal LH., Entman ML. (1999) Histochemical and morphological characteristics of canine cardiac mast cells. Histochem J 31(4):221-229

Product List

Product Name Cat# Quantity Price

Anti Macrophage Surface Antigen

KAL-KT013 50UG

¥ 49,000
$ 654
€ 490