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useful antibody for the study of MSR-A in atherogenesis and various other pathological conditions.

Anti Human Macrophage Scavenger Receptor A (MSR- A:CD204) Monoclonal Antibody (Clone No. SRA-C6)

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Background

Class A macrophage scavenger receptor (MSR-A: CD204) was identified in the search for the receptor molecules that are implicated in the pathological deposition of cholesterol during atherogenesis through receptor-mediated uptake of modified low density lipoprotein (LDL). MSR-A possesses a wide range of ligand-binding specificities and recognize a variety of molecules such as modified LDL including acetylated LDL, oxidized LDL, advanced glycation end products (AGEs), polyribonucleotides such as poly G and poly I and bacterial surface lipids including lipopolysaccharide and lipoteicoic acid.
This antidody was produced from the mouse immunized with recombinant protein of human type I MSR-A ,and has been proved to be useful for the western blotting and immunohistochemistry. This antibody also inhibits the endocytic degradation of acetylated LDL and oxidized LDL by high glucose-treated human monocyte-derived macrophages and has anti MSR-A neutralizing activity.
This antibody is useful tools for the study of MSR-A in atherogenesis and various other pathological conditions.

Package Size 50μg (200μl / vial)
Format
Buffer
Mouse monoclonal antibody 0.25mg/ml
PBS [containing 2% Block Ace as a stabilizer, 0.1%Proclin as a bacteriostat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. SRA-C6
Subclass IgG1
Purification method The spleen cells obtained from MSR-A deficient mouse, immunized with recombinant protein corresponding to amino acid 131-451 of human type I MSR-A, were fused with mouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography..
Working dilution for  immunohistochemistry: 5.0μg/ml, western blotting : 1.0μg/ml,
Neutralization: Depends on the experimental design(Application Reference:1)


Human alveolar macrophages
(Cytospin preparation)
: Most macrophages are positive


Human liver (paraffin section)
: Kupffer cells are positive

Neutralizing activity of SRA-C6 (20μg/ml)
: Inhibitory effect of anti-human SR-A antibody on the degradation of 125I-AcLDL by human monocyte-derived macrophages(day7)

Takeya M., Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan

Product List

Product Name Cat# Quantity Price

Anti Macrophage Scavenger Receptor A

KAL-KT118 50UG

¥ 55,000
$ 734
€ 550

Anti Macrophage Scavenger Receptor A (additive-free)

KAL-KT118-S 50UG

¥ 55,000
$ 734
€ 550