Macrophages are present in nearly all tissues and organs of the body. They are differentiated from monocytes derived from the bone marrow. Macrophages and monocytes are phagocytes, acting in both innate immunity and cell-mediated immunity of vertebrate animals. Their function is to phagocytize cellular debris and pathogens , and to stimulate lymphocytes and other immune cells to respond to the pathogen.
Macrophages are able to be identified immnohistochemically by virtue of the presence of monocyte/macrophage-associated antigens such as CD68. Antibodies recognizing CD68 have been used as some of the best reagents to detect macrophages in tissues.
CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family.
This antibody recognizes CD68 antigen. The molecular size of the antigen identified by this antibody was 110 kDa. Immunopecipitated antigen by this antibody was also recognized by the other CD68 antibodies such as KP-1 and PG-M1.
In immunohistochemical assays, this antibody recognizes freshly isolated human blood monocytes and tissue macrophages. This antibody also recognizes macrophages obtained from guinea pigs, pigs, bovine species, and monkeys. Since this antibody strongly labels guinea pig macrophages, this antibody will be suitable to examine such macrophages in experimental guinea pig models.
This antibody will be very useful to research of CD68, macrophage, allergic diseases and delayed hypersensitivity.
|Package Size||50μg (200μL/ vial)|
|Format Buffer||Mouse monoclonal antibody 0.25mg/mL
PBS [containing 2% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
|Storage||Store below 20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
|Purification method||The spleen cells from BALB/c mouse, immunized with human peritoneal cells from patients with endometriosis incubated for 24 hours, were fused with mouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.|
|Working dilution for immunohistochemistry: 10μg/mL|
Human spleen (frozen section):Red pulp macrophages are positive
Takeya M., Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
|Heart||Intramuscular Mφ||Lymph nodes||Mφ in follicles
Mφ in paracortical areas
|Lung||Alveolar Mφ||Pancreas, Salivary, Thyroid,
Adrenals, Urinary bladder,
Stomach, Small and Large intestines Mφin lamia propria
Mφ in portal triads
Uriniferous tubule cells
|Blood monocytes||Freshly isolated monocytes|
|Spleen||Red pulp Mφ
White pulp Mφ
|Blood neutrophils||Freshly isolated neutrophils|
|Thymus||Mφ in cortex
Mφ in medulla
|Myeloid cell lines||THP-1
Mφ : macrophage
Anti Macrophage / CD68