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Useful for monitoring the expression of HLA-A, -B, and -C in formalin-fixed, paraffin embedded sections and also useful for western blotting.

Anti human HLA class I (HLA-A,B,C) monoclonal antibody

Background

Class I major histocompatibility complex proteins (MHC class I) are present on the surface of virtually all nucleated vertebrate cells and play a central role in immune surveillance (self/non-self recognition). On the cell surface, MHC class I molecules are composed of one membrane bound heavy chain (alpha chain) bound non-covalently to beta-2 microglobulin. MHC class I proteins function to present antigens to CD8+ T cells and form ligands for receptors on natural killer (NK) cells. Expression levels of MHC class I molecules may vary greatly in response to viral infection, neoplasia, and other abnormal conditions.
In humans, MHC molecules are referred to as HLA (human leukocyte antigens). The class I human HLA isotypes HLA-A, HLA-B, and HLA-C are the most polymorphic of the six human class I isotypes. Clone EMR8-5 recognizes a non-polymorphic determinant present on all on HLA-A, -B, and -C heavy chains that is stable following formalin fixation and is thus the first antibody useful for monitoring the expression of HLA-A, -B, and -C in formalin-fixed, paraffin embedded sections. Clone EMR8-5 is also useful for western blotting.

Application

[western blotting]
Fig1 Human tumor cell line
  1. Oral cavity cancer cell line OSC20
  2. OSC20-A2042 (HLA-A*2402 transfectant)
  3. Human leukemia cell line K562
    (no appearance the gene HLA class )
  4. Recombinant HLA-A*2402 heavy chain protein
Fig.2 Recombinant HLA-A,B,C heavy chain
 
[Immnohistchemistry]

<Reference>
1. Torigoe T, Asanuma H, Shimozawa K, et al. Immunohistochemical analysis of HLA class I expression in tumor tissues revealed unusually high frequency of down-regulation in breast cancer tissues. J. Translational Med. Submitted
2. Komori H, Nakatsura T, Senju S, Yoshitake Y, Motomura Y, Beppu T, Matsui M, Torigoe T, Sato N, Baba H, Nishimura Y. Identification of HLA-A2 or A24-restricted CTL epitopes possibly useful for glypican-3-specific immunotherapy for hepatocellular carcinoma.
Clinical Cancer Res. 12,2689-2699(2006)
3. Kitamura H, Torigie T, Sato N,et.al. Expression and antigenecity of surviving, an inhibitor of apotosis family member, in bladder cancer : implications for specific immunotherapy.
J Urology. Submitted.67,955-959(2006)

Product List

Product Name Cat# Quantity Price

Anti HLA class I-A, B, C

HKD-AB-46 100UL

¥ 60,000
$ 800
€ 600

Anti HLA class I-A,B,C

HKD-AB-46-B 100UL

¥ 80,000
$ 1067
€ 800

Anti HLA class I-A,B,C

HKD-AB-46-F 100UL

¥ 80,000
$ 1067
€ 800

Anti HLA class I-A,B,C

HKD-AB-46-H 100UL

¥ 80,000
$ 1067
€ 800