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This antibody is specific to GPR120 and will be useful for research on diabetes, obesity

Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6)

Background

GPR120 is a member of G protein-coupled receptor (GPCR) family and functions as a specific receptor for unsaturated long-chain free fatty acids (FFAs) such as palmitoleic acid, α-linolenic acid, docosahexaenoic acid. The expression of GPR120 is widely detected in various tissues, and GPR120 is highly expressed in the pituitary, lung, small intestine, colon, and adipose tissues. It has been shown that the stimulation of GPR120 by FFAs promotes the secretion of GLP-1, known as the most potent insulinotropic incretin, from the gastrointestinal tract. It has been also demonstrated that FFAs stimulate the ERK and PI3K-Akt pathways through GPR120 and lead to anti-apoptotic effect in enteroendocrine cell line, and that GPR120 regulates adipogenic process such as adipocyte development and differentiation. In addition, a study suggested that GPR120 is expressed in the taste bud cells in circumvallate papillae to sense dietary fat. This antibody is specific to GPR120 and will be useful for research on diabetes, obesity.

Application

WB Not tested
IHC Not tested
ICC 5.0 μg/mL
ELISA Not tested
FCM 1.0-5.0 μg/mL
Neutralization Not tested
IP Not tested

Immunocytochemical staining of HEK293T cells overexpressing human GPR120

Character

Primary Source HGNC: 19061
Type Monoclonal
Immunogen Partial peptide of Human GPR120
Raised in GANP mouse
Myeloma P3U1
Clone number 2B6
Isotype IgG2aκ
Source Serum-free conditioned medium
Purification notes ProteinG
Cross Reactivity Not yet tested in other species.
Concentration 0.25 mg/mL
Contents(Volume) 25 μg(100 μL/vial)
Label Unlabeled
Buffer PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]
Storage Store below 20 °C. Once thawed, store at 4 °C. Repeated freeze-thaw cycles should be avoided.

This product is generated from GANP mice

Product List

Product Name Cat# Quantity Price

Anti GPR120

KAL-KG138 25UG

¥ 55,000
$ 734
€ 550

References
  • Gotoh C. et al.: The regulation of adipogenesis through GPR120.
    Biochem Biophys Res Commun. 2007 Mar 9;354(2):591-7. Epub 2007 Jan 16
  • Matsumura S. et al.: GPR expression in the rat taste bud relating to fatty acid sensing.
    Biomed Res. 2007 Feb;28(1):49-55
  • Katsuma S. et al.: Free fatty acids inhibit serum deprivation-induced apoptosis through GPR120 in a murine enteroendocrine cell line STC-1.
    J Biol Chem. 2005 May 20;280(20):19507-15. Epub 2005 Mar 17
  • Hirasawa A. et al.: Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120.
    Nat Med. 2005 Jan;11(1):90-4. Epub 2004 Dec 26