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Anti Collagen 4

Anti Human Collagen IV α Chain Monoclonal antibodies

Background

Eptope-defined monoclonal antibodies against the NC1 domains of α1 - α6 (IV) chains of type-IV collagen are against the triple-helical domain. These epitope-defined monoclonal antibodies against different site of the collagen mokecule are useful for research on type-IV collagen and diagnosis of hereditary disease related to this collagen because of their reliability.

Clone H25 is specific to imperfection XIII of human α2(IV). (Ref. 1)
Clone B51 is specific to NC1 domain of human α5(IV). Not reactive with human α1, α2, α3, α4 and α6.(Ref. 2)
Clone H53 is specific to imperfection III of human α5(IV). Not reactive with human α1, α2, α3, α4 and α6. Epitope is IDVEF. (Refe. 1)
Clone H52 is specific to NC1 domain of human α5(IV). Not reactive with human α1, α2, α3, α4 and α6. Epitope is SKPQSETL. Reactive with NC1 domain of α5(IV) of rat, rabbit, guinea pig, dog and bovine. (Ref. 1)

clone Cat # label
H25 #SGE-C425
non
#SGE-CFT425 Texas Red
B51 #SGE-C451
non
#SGE-CFT451 FITC
H53 #SGE-C453
non
#SGE-CFT453 FITC
H52 #SGE-C452 non

Reference
1) Kagawa M, Kishiro Y, Naito I, Nemoto T, Nakanishi H, Ninomiya Y, Sado Y., Nephrol Dial Transplant. 1997 Jun;12(6):1238-41. PMID: 9198058
2) Borza DB, Bondar O, Ninomiya Y, Sado Y, Naito I, Todd P, Hudson BG., J Biol Chem. 2001 Jul 27;276(30):28532-40. PMID: 11375996

Application

Anti Human Collagen IV α5(IV) Chain Rat Monoclonal antibody clone 52(#SGE-C-452) can be used for immunostaining of cryostat sections and for western blotting
How to stain human cryostat sections with #SGE-C-452 (clone H52).
Materials
  1. Acid solution : KCl-HCl buffer, pH 1.5, Ion strength=0.1 (Mix equal volumes of 0.1 M KCl and 0.1 M HCl.)
  2. Blocking solution: 10% goat serum (normal goat serum diluted with PBS)
  3. First antibody: H52 monoclonal Ab is usually diluted to 1/10 - 1/100 with PBS containing 1 mg/ml of bovine serum albumin before use.
  4. Secondary Ab: Affinity purified Ab to rat IgG (Cappel #55760) is usually diluted to 1/50- 1/100 with PBS containing 5% human normal serum before use.
Procedure
  1. Cryostat sections: 4 µm
  2. Treatment with acetone for 10 min
  3. Acid-solution treatment with 0.1 M KCl- HCl (pH1.5) for 10 min
  4. Washing with PBS
  5. Blocking with 10% normal goat serum for 10 min
  6. Washing
  7. First Ab for 60 min
  8. Washing
  9. Secondary Ab for 30 min
  10. Washing
  11. Mount with Perma Fluor (Tanner #11611)

Human cryostat section stained with monoclonal antibody H52.
Glomerular basement membrane (BM), part of tubular BM, and Bowman’s capsular BM are stained.


 Collagen Detection Antibodies and Kits Flyer [PDF]
  1. Cell Matrix Protein Antibody (Anti Collagen)
  2. Cell Matrix Protein Antibody (Others)
  3. Bone Marker Protein Antibody
  4. Visual Protein Antibody
  5. Enzyme Antibody
  6. Homeobox Protein Antibody
  7. anti human collagen IV α chain antibodies
  8. anti collagen 7 monoclonal antibody
  9. Alport's Syndrome Monoclonal Staining Cocktail

Product List

Product Name Cat# Quantity Price

Anti Collagen 4 (ALPHA)5(IV)

SGE-C453 500UL

¥ 28,000
$ 374
€ 280

Anti Collagen 4 (ALPHA)2(IV)

SGE-C425 500UL

¥ 28,000
$ 374
€ 280

Anti Collagen 4 (ALPHA)5(IV)

SGE-C451 500UL

¥ 28,000
$ 374
€ 280

Anti Collagen 4

SGE-C-452 500UL

¥ 28,000
$ 374
€ 280

Anti Collagen 4 alpha5(IV)

SGE-CFT453 500UL

¥ 32,000
$ 427
€ 320

Anti Collagen 4 (ALPHA)2(IV)

SGE-CFT425 500UL

¥ 32,000
$ 427
€ 320

Anti Collagen 4 (ALPHA)5(IV)

SGE-CFT451 500UL

¥ 32,000
$ 427
€ 320

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.