CD98 (also known as 4F2) is widely expressed cell surface around 120-kDa heterodimetric protein. CD98 is composed a common type II transmembrane heavy chain of around 80-kDa (also kwon as CD98hc, 4F2hc or SLC3A2) and one of at least six light chains of around 40-kDa (LAT1, LAT2, y+LAT1, y+LAT2, xCT, asc) to form amino acid transporters. It is thought that the heavy chain is mainly involved in the trafficking of the complex to the plasma membrane, whereas the light chain catalyses the transport itself. CD98hc is a multifunctional glycoprotein, and was originally discovered as a T-cell activation antigen. CD98hc is highly expressed on proliferating lymphocytes, tumor cells and on the other rapidly-growing cells. It has also shown that CD98hc has an important role in integrin-dependent signals that promote tumorigenesis. This antibody is specific to human CD98hc and will be useful for FCM, immunoprecipitation.
|Immunogen||Human Cancer Cell Line|
|Source||Serum Free Medium|
|Cross Reactivity||Not yet tested in other species.|
|Contents(Volume)||50 μg (200 μL/vial)|
|Buffer||PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]|
|Storage||Store below 20 °C. Once thawed, store at 4 °C. Repeated freeze-thaw cycles should be avoided.|
1. Nakamura E. et al.: 4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer. J Biol Chem.1999 Jan 29;274(5):3009-16.
2. Nii T. et al.: Molecular events involved in up-regulating human Na+-independent neutral amino acid transporter LAT1 during Tcell activation. Biochem J. 2001 Sep 15;358(Pt 3):693-704.
3. Fenczik CA. et al.: Distinct domains of CD98hc regulate integrins and amino acid transport. J Biol Chem. 2001 Mar 23;276(12):8746-52. Epub 2000 Dec 19.
4. Henderson NC. et al.: CD98hc (SLC3A2) interaction with beta 1 integrins is required for transformation.
J Biol Chem.2004 Dec 24;279(52):54731-41. Epub 2004 Oct 12.
5. Feral CC. et al.: CD98hc (SLC3A2) mediates integrin signaling. Proc Natl Acad Sci U S A.2005 Jan 11;102(2):355-60. Epub 2004 Dec 29.
Anti CD98hc / 4F2