Na/K-ATPase is a membrane-associated enzyme responsible for the active transport of Na + and K+, and the maintenance of Na+ and K+ gradients across membrane. It is a heterodimer of two different kinds subunit, a large α subunit and a smaller glycoprotein subunit β. At least four distinct α subunit and four β subunit isoforms have been identified. In some tissues, it has been showed that Na/K-ATPase is associated with third subunit γ, a small regulatory protein.
CD298 (also known as Na/K-ATPase β3) is a 42-kDa glycoprotein and one of β subunit isoforms. CD298 has been detected in amphibians, mouse, rat, and human. In rat, CD298 expresses in a variety of tissues including testis, brain, kidney, spleen, stomach, small intestine, colon, lung, and liver. In the retina Na/K-ATPase restores Na+ and K+ gradients used by photoreceptor dark current, synaptic activity, action potential, and CD298 is largely restricted to photoreceptors in the mouse and rat. In human, CD298 also involves in regulation of T and B lymphocyte proliferation. This antibody is specific to human CD298 and will be useful for FCM, immunoprecipitation, immunohistochemistry.
|Immunogen||Human Cancer Cell Line|
|Source||Serum Free Medium|
|Cross Reactivity||Not yet tested in other species.|
|Contents(Volume)||50 μg(200 μL/vial)|
|Buffer||PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]|
|Storage||Store below 20 °C. Once thawed, store at 4 °C. Repeated freeze-thaw cycles should be avoided.|
1. Malik N. et al.: Identification of the mammalian Na,K-ATPase beta3 subunit. J Biol Chem.1996 Sep 13;271(37):22754-8.
2. Arystarkhova E. et al.: Tissue-specific expression of the Na,K-ATPase beta3 subunit. The presence of beta3 in lung and liver addresses the problem of the missing subunit. J Biol Chem.1997 Sep 5;272(36):22405-8.
3. Blanco G. et al.: Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function. Am J Physiol.1998 Nov;275(5 Pt 2):F633-50. Review.
4. Wetzel RK. et al.: Cellular and subcellular specification of Na,K-ATPase alpha and beta isoforms in the postnatal development of mouse retina. J Neurosci. 1999 Nov 15;19(22):9878-89.
5. Chiampanichayakul S. et al.: Engagement of Na,K-ATPase beta3 subunit by a specific mAb suppresses T and B lymphocyte activation.
Int Immunol.2002 Dec;14(12):1407-14.