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useful for Western blotting or ELISA for HIV-1 reverse transcriptase detection and titration.

Anti HIV-1 Reverse Transcriptase Polyclonal Antibody

Background

HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1(AIDS virus), subtype B origin (1). It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus. The product is prepared by immunizing rabbit with HIV-1 reverse transcriptase which was uniquely over-overexpressed as recombinant protein in E. coli by a patent method and highly purified (2). Using this antiserum in Western blotting, the bands of 66 kD and 51 kD corresponding respectively to the two subunits of HIV-1 reverse transcriptase were observed in the extract of the AIDS virus infected cells (Fig 1).

 

Fig. 1 Detection of HIV-1 reverse transcriptase
by Western blotting using anti-HIV-1 reverse
transcriptase antibody
Lane 1, Extract of MT4 cells.
Lane 2, Extract of MT4 cells infected
with HIV-1 (LAI strain).
The antiserum was diluted 2500 fold before use.

 

<Reference>
1. Adachi A, et al., J. Virol. 59, 284 (1986)
2. Saitoh A, et al., Microbiol. Immunol. 34:509-521 (1990)

Product List

Product Name Cat# Quantity Price

Anti HIV-1 Reverse Transcriptase

BAM-65-001-EX 50UL

¥ 30,000
$ 400
€ 300

Anti HIV-1 Reverse Transcriptase

BAM-65-002-EX 250UL

¥ 90,000
$ 1200
€ 900

Anti HIV-1 Reverse Transcriptase

BAM-65-003-EX 50UL

¥ 30,000
$ 400
€ 300