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useful for Western blotting or ELISA for HIV-1 Gag p17 detection and titration.

Anti HIV-1 Gag p17 Polyclonal Antibody

Background

HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease.  This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the of AIDS virus particle construction (1).
The product is prepared by immunizing rabbit with recombinant p17 protein which was over-expressed in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3,4).
Using this antiserum in Western blotting, the bands of 17 kD and 55 kD corresponding respectively to HIV-p17 and to its precursor p55 were observed in the extract of the AIDS virus infected cells (Fig. 1)

 

Fig. 1 Detection of HIV-1 p17 and its p55 precursor proteins by Western blotting using the p17 antibody.
Lane 1, Extract of MT4 cells.
Lane 2, Extract of MT4 cells infected with HIV-1(LAI strain).
The antiserum was diluted 2500 fold before use.

 

<Reference>
1. Freed EO, Virology 251:1-15 (1998) Review
2. Adachi A, et al., J. Virol. 59, 284 (1986)
3. Saitoh A, et al., Microbiol. Immunol. 36:105-111 (1992)
4. Saito A, et al., Microbiol. Immunol. 39:473-483 (1995)

Product List

Product Name Cat# Quantity Price

Anti HIV-1 Gag p17

BAM-65-008-EX 50UL

¥ 30,000
$ 400
€ 300

Anti HIV-1 Gag p17

BAM-65-009-EX 250UL

¥ 90,000
$ 1200
€ 900

Anti HIV-1 Gag p17

BAM-65-010-EX 50UL

¥ 30,000
$ 400
€ 300

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.