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useful for the research of MCM3/GANP complex phosphorylation state during cell cycle progression

Anti G5PR polyclonal antibody

Background

G5PR was cloned as novel protein which could bind to Germinal center Associated DNA Primase(GANP). GANP is involved in important events that regulate DNA replication in activated germinal centre B cells. G5PR is homologous to known regulatory B’’ subunits of PP2A, and the G5PR protein is associated with PP5 and PP2A, suggesting that G5PR is a GANP-associated PPase component. G5PR contributes to the regulation of the phosphorylation state of the chromatin-bound or nonbound form of MCM3 (minichromosome replication molecule 3) in vivo. So that, G5PR might regulate MCM3/GANP complex phosphorylation state during cell cycle progression.
This antibody is very useful for immunoprecipitation and westernblotting.

Application

Working dilution for immunoprecipitation : 2 μg
Working dilution for western blotting : 1〜5 μg/mL

Westernblotting
Sample : Myc-GFP-G5PR co-transfected cell lycate
? Immunoprecipitated by anti-G5PR antibody, then ditected by anti-Myc antibody
?Immunoprecipitated by anti-Myc antibody, then ditected by anti-G5PR antibody

Preparation of antibodies and instruction
Drs.Sakaguchi N. and Igarashi H. at Department Immunology, Kumamoto University School of Medicine

Character

Package Size 25μg (100μL / vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Below -20°C
Purification method This antibody was purified from rabbit serum by affinity chromatography.

<Reference>
1. Genes Cells 2002 Aug;7(8):821-34

Product List

Product Name Cat# Quantity Price

Anti G5PR

KAL-KR058 25UG

¥ 49,000
$ 654
€ 490

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.