Polymeraseδis one of the three essential DNA polymerases of eukaryotes for chromosome replication, and also involved in nucleotide excision repair, base excision repair and VDJ recombination (1, 2). p66 is a functionally important subunit of human polymeraseδ, stabilizes polymeraseδ complex and increases the affinity of polymeraseδfor PCNA (3).
This product is the IgG fraction purified from serum-free culture medium of mouse hybridoma (2A1C11) by propriety chromatography under mild conditions.
1) Western blotting
Fig. Detection of p66 protein of polymerase δ by Western blotting.
1. Hindges R and Hubscher U Biol. Chem. 378, 345-362 (1997)
2. Johnson A and O’Donnell M. Annu Rev Biochem 74: 283 (2005)
3. Shikata K et al. J. Biochem. 129, 699-708 (2001)
Anti DNA polymerase (DELTA) subunit p66