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useful for investigating TRPM8 (CMR1) expression pattern and analyzing the function.

Anti Cold/menthol receptor1(TRPM8) Polyclonal antibody

For Research Use Only

Background

All mammalians including human being can detect the temperature by their own skin. Recently, several molecular mechanism has been proposed to explain physiological stimuli. For example capsaicin receptors, VR-1/TRPV1and VRL-1/TRPV2, which are related with noxious stimuli were cloned and found that these molecules were activated by temperatures exceeding 43°C and exceeding 50°C.
Jullus et al. and Patapoutian et al. found novel receptor, cold and menthol-sensitive receptor 1 (CMR1)/TRPM8, and reported to Nature and Cell in which they described that these molecules were activated in temperature range 8~28°C. These findings might become driving force to investigate the mammalian peripheral nervous system.So that, in near future, the advantage of molecular physiology could account for thermal stimuli such as 1)Why human can distinguish between cold and warm? 2)Why the nervous activity accompanied by cold sensation does not always induce an unpleasant feeling or pain?
This antibody is very useful for investigating cold/menthol receptor1 (TRPM8) expression pattern and analyzing the function.

Package Size 25g (100L/vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1%Proclin as bacteriostat
Storage Store below 20°C.
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was purified from rabbit serum by affinity chromatography.
Working dilution Immunohistochemistry : 2 ~5g/mL

Immunohistochemistry
Sample:Rat dorsal root neuron
             Positive : Green

Preparation of antibodies and instruction
Dr.Makoto Tominaga at Department of Physiology,Mie University School of Medicine

<Reference>
1. Story GM, Peier AM, Reeve AJ, Eid SR, Mosbacher J, Hricik TR, Earley TJ, Hergarden AC, Andersson DA, Hwang SW, McIntyre P, Jegla T, Bevan S, Patapoutian A. ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell. 2003 Mar 21;112(6):819-29.
2. Nealen ML, Gold MS, Thut PD, Caterina MJ. TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat. J Neurophysiol. 2003 Mar 12
3. Zemelman BV, Nesnas N, Lee GA, Miesenbock G. Photochemical gating of heterologous ion channels: remote control over genetically designated populations of neurons. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1352-7.
4. Guler AD, Lee H, Iida T, Shimizu I, Tominaga M, Caterina M. Heat-evoked activation of the ion channel, TRPV4. J Neurosci. 2002 Aug 1;22(15):6408-14.
5. Xu H, Ramsey IS, Kotecha SA, Moran MM, Chong JA, Lawson D, Ge P, Lilly J, Silos-Santiago I, Xie Y, DiStefano PS, Curtis R, Clapham DE. TRPV3 is a calcium-permeable temperature-sensitive cation channel. Nature. 2002 Jul 11;418(6894):181-6.
6. Peier AM, Moqrich A, Hergarden AC, Reeve AJ, Andersson DA, Story GM, Earley TJ, Dragoni I, McIntyre P, Bevan S, Patapoutian A. A TRP channel that senses cold stimuli and menthol. Cell. 2002 Mar 8;108(5):705-15.
7. McKemy DD, Neuhausser WM, Julius D. Related Identification of a cold receptor reveals a general role for TRP channels in thermosensation. Nature. 2002 Mar 7;416(6876):52-8.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.