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useful for potrcial role recerch of AGEs modification in nomal aging as well as age-enhanced desease processes

Anti CEL [Nε-(carboxymethyl) lysine] Monoclonal Antibody

Background

Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE). Recent immunological studies using anti-AGE antibody (6D12) demonstrated the presence of AGE-modified proteins in several human tissues: (1) human lens (nondiabetic and noncataractous), (2) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (3) diabetic retina, (4) peripheral nerves of diabetic neuropathy, (5) atherosclerotic lesions of arterial walls, (6)β2-microglobulin forming amyloid fibrils in patients with hemodialysis-related amyloidosis, (7) senile plaques of patients with Alzheimer’s disease, (8) the peritoneum of CAPD patients, (9) skin elastin in actinic elastosis, and (10) ceriod/lipofuscin deposits. These results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease processes. This antibody named as 6D12 has been used to demonstrate AGE-modified proteins in these human tissues, indicating potential usefulness of this antibody for histochemical identification and biochemical quantification of AGE-modified proteins.

CEL is known to generate from protein modification by methylglyoxal . Mclellan et al. demonstrated that plasma methylglyoxal, which is believed to be generate from Embden-Meyerhof and polyol pathways, concentrations in insulin-dependent diabetic patients were about 7-times higher than those of normal individuals. For examples, CEL was identified in human lens proteins at a concentration similar to that of CML and its accumulation increased with age like CML, indicating that CEL may play an important marker for aging and age-dependent disease such as diabetic complications.

 AGEs Antibody Flyer [PDF]
 AGEs Antibody Flyer (print file) [PDF]
 
Package Size 50µg  (200µL/vial)
Format
Buffer
Mouse monoclonal antibody 0.25 mg/mL
Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below  -20°C.
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. KNH-30
Subclass IgG1
Purification method The splenic lymphocytes from BALB/c mouse, immunized with CEL-BSA were fused to myeloma P3U1 cells. The cell line (KNH-30) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 5-10µg/mL; for ELISA: 0.1-1.0µg/mL
References for CEL
Ahmed MU,et al., Biochem J. 1997 Jun 1;324 ( Pt 2):565-70. PMID: 9182719
Degenhardt TP,et al.,Cell Mol Biol (Noisy-le-grand). 1998 Nov;44(7):1139-45. PMID: 9846896
Mclellan AC,et al.,Clin Sci (Lond). 1994 Jul;87(1):21-9. PMID: 8062515

*These references are the background of CEL , and are not this antibody examples

References for AGEs antibody
1 Horiuchi S,et al., J Biol Chem. 1991 Apr 25;266(12):7329-32. PMID: 2019568
2 Araki N,et al., J Biol Chem. 1992 May 25;267(15):10211-4. PMID: 1587810
3 Miyata T,et al., J Clin Invest. 1993 Sep;92(3):1243-52 PMID: 8376584
4 Yamada K,et al., Clin Nephrol. 1994 Dec;42(6):354-61. PMID: 7882598
5 Kume S,et al., Am J Pathol. 1995 Sep;147(3):654-67. PMID: 7545874
6 Makino H,et al., Kidney Int. 1995 Aug;48(2):517-26. PMID: 7564121
7 Mori T,et al., Exp Mol Pathol. 1995 Oct;63(2):135-52. PMID: 8941048
8 Miyata T,et al., Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2353-8. PMID: 8637877
9 Kimura T,et al., Neurosci Lett. 1996 Apr 12;208(1):53-6. PMID: 8731173
10 Ikeda K,et al., Biochemistry. 1996 Jun 18;35(24):8075-83. PMID: 8672512

Product List

Product Name Cat# Quantity Price

Anti CEL

KAL-KH025 50UG

¥ 55,000
$ 734
€ 550

Anti CEL

KAL-KH025-01 50UG

¥ 70,000
$ 934
€ 700

Anti CEL

KAL-KH025-02 50UG

¥ 70,000
$ 934
€ 700