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Anti Cdc48 Antibody

Background

Involved in spindle disassembly, degradation of ubiquitinated proteins and protein export from the endoplasmic reticulum to the cytoplasm. Acts as a chaperone that collects ubiquitinated substrates. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1. Has an additional role in the turnover of OLE1 where it targets ubiquitinated OLE1 and other proteins to the ERAD. Cdc48 consists of 836 amino acids with molecular mass of 92 kDa.

Application

Western blotting (1/500~1/5,000 dilution). Not tested for other applications.

Identification of endogenous Cdc48 protein in crude extract of S. cerevisiae . Total protein (40 μg) of S. cerevisiae strain W303 was analyzed by western blot. 1. Anti-Cdc48 antibody at 1/500. 2. Anti-Cdc48 antibody at 1/2,000 3. Anti-Cdc48 antibody at 1/5,000 As second antibody, HRP-conjugated anti-rabbit IgG was used at 1/10,000 dilution. Positions of molecular size markers were shown in kDa on the left. Cdc48 calculated from amino acids sequence is 92 kDa

Product List

Product Name Cat# Quantity Price

Anti Cdc48

BAM-62-303-EX 100UL

¥ 42,100
$ 562
€ 421

References
  • Noguchi M, et al. ATPase activity of p97/valosin-containing protein is regulated by oxidative modification of the evolutionally conserved cysteine 522 residue in Walker A motif. J Biol Chem. 2005 Dec 16;280(50):41332-41. Epub 2005 Oct 18. PMID: 16234241
  • Takata T,et al., : Rescue of growth defects of yeast cdc48 mutants by pathogenic IBMPFD-VCPs. J Struct Biol. 2012 Aug;179(2):93-103. doi: 10.1016/j.jsb.2012.06.005. Epub 2012 Jun 19. PMID: 22728077

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.