The novel zebrafish protein Ccd1 (Coiled-coil-DIX1) possesses a C-terminal DIX (Dishevelled-Axin) domain as well as an N-terminal coiled-coil domain. The DIX domain proteins Ccd1, Axin, and dishevelled (Dvl / Dsh) are important in Wnt signaling. Ccd1 forms a heteromeric complex with Axin and Dvl/Dsh and regulates neural patterning through Wnt pathway activation. This antibody presented here reacts with the coiled-coil domain of the Ccd1 isoforms Ccd1A, Ccd1B, Ccd1C.
(Brain Res Mol Brain Res., 2005 Apr 27; 135(1-2):169-80).
A:Zebrafish Ccd1-transfected Hela cells
B:Mouse Ccd1B-transfected Hela cells
Preparation of antibodies and instruction:
Masu M. Shiomi K.
University of Tsukuba
Graduate School of Comprehensive Human Sciences
|Package Size||25g (100L/vial)|
|Format||Rabbit polyclonal antibody (0.25mg/mL)|
|Buffer||PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteriostat]|
|Storage||Store below -20C
Once thawed, store at 4C. Repeated freeze-thaw cycles should be avoided.
|Purification method||This antibody was established from the serum of a rabbit immunized with a peptide fragment of Ccd1.
Purified by peptide affinity chromatography.
|Working dilution||For Western blotting : 1.0g/ml
For Immnocytochemistry : 1.0〜2.0g/ml