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The first in the world! An antibody which detects Catechin!

Anti Catechin

The world's first "Catechin antibody" is launched from Cosmo Bio and can be used for assays or quantification of Catechin.






See Also    Unique Plant Antibodies Developed by Kyusyu University

 

Background

Catechins are one type of polyphenol included in green tea, known to show multimodal effects such as antioxidant, anticancer, anti-allergic, antibacterial and abdominal fat reducing effects. Our antibody reacts with epigallocatechin (EGC), epicatechin gallete (ECg), epigallocatechin gallete (EGCg), gallocatechin (GC), catechin gallete (Cg) and gallocatechin gallete (GCg). It especially reacts well with gallocatechin gallete (GCg) and epigallocatechin gallete (EGCg).

 

Application

This antibody reacts strongly with an epicatechin gallate(ECg), gallocatechin gallate(GCg), catechin gallate(Cg)、epigallocatechin gallate(EGCg), gallocatechin(GC).

Please see application of Direct ELISA(Figure 1)、SPR measurement (Figure 2).

 

Direct ELISA法にてan epicatechin gallate(EGCg)とepicatechin(EC)の濃度を測定
Figure1. Direct ELISA data of EGCg and EC with Anti Catechin monoclonal Antibody (Clone : b-1058)
Anti Catechin (Clone:b-1058), x10,000 dilution(0.1ug/mL), 50uL were added.

 

SPR測定にて抗カテキン抗体(クローン:b-1058)とan epicatechin gallate(EGCg)とepicatechin(EC)の親和性を測定
Figure 2. Evaluation of EGCg/EC-Catechin monoclonal antibody (Clone : b-1058) interaction was performed by BIAcoreX.
The b-1058 was diluted in 10mM acetate buffer, pH 5.0 and bind on CM5 sensor chip. Binding of EGCg/EC was determined a range of concentrartion (0.012 to 100ug/mL) in 0.1M phosphate buffer, pH6.0 containing HBS-EP buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20) with a flowrate of 10uL/min at 25℃.

Details

Product Anti Catechin  
Antigen 3-Succinyl Epicatechin (EC) conjugated KLH  
immune animal Mouse (BALB/c) Clone No. b-1058
Isotype IgG2a Origin Ascite
Purification Ion exchange chromatography  
Buffer Phosphate buffered saline (PBS)
*NOTE:PBS doesn't contain preservative. Preservative is added based on the research purpose  
Concentration 1 mg / mL   
Label Unlabeled   
Cross reactivity ECg, Cg, GCg, EGCg, GC and EGC
Minimal cross reactivity with catechin and epicatechin
Volume 100 uL
Storage below -20 degrees  
Application

・direct ELISA 1 / 10,000 (0.1ug / mL)
・SPR 57ug / mL

Other applications have not been tested. Optimal dilutions/concentrations should be determined by the end user.  

Product List

Product Name Cat# Quantity Price

Anti Catechin

CAC-KYU-TM-M001 100UG

¥ 40,000
$ 534
€ 400

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.