Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Fig.1. Western blotting analysis of mouse testis extracts of different ages with anti-Calnexin (CANX) antibody.
20 µg of Triton X-100 extracts from mouse testes was reacted with anti-Calnexin antiserum at 1/1,000 dilution. Calnexin expression started at birth. (wks) stands for weeks.
Fig.2. Immunoprecipitation of Calnexin from mouse testis.
One mg of testis lysate (supernatant in lysis buffer containing 10 mM Tris-HCl pH8.0, 50 mM NaCl, 1% protease inhibitor mix) was incubated with 2 µl of anti-Calnexin (CANX) antiserum and 50 µl. of protein-A conjugated magnetic beads (Miltenyi Biotec) and immunoprecipitated according to the protocol of supplier. The immunoprecipitated sample was analyzed by western blotting using the same antibody at 1/1,000 dilution.
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