Calmegin plays an important role in sperm fertility. Binds calcium ions. Specifically expressed during male meiotic germ cell development.
Fig.1. Western blotting analysis of testis extracts of wild-type and knockout mice with anti-Calmegin antibody. 20 µg of Triton X-100 extracts from mouse testes was reacted with anti-Calmedin antiserum at 1/1,000 dilution. Arrow indicates the position of intact Calmegin.
Fig.2. Immunoprecipitation of Calmegin from mouse testis. One mg of testis lysate was incubated with 2 µl of anti-Calmegin antiserum and 50 µl. of protein-A conjugated magnetic beads (Miltenyi Biotec) and immunoprecipitated according to the protocol of supplier. The immunoprecipitated sample was analyzed by western blotting with the antibody at 1/1,000 dilution. 1. Input 2. non-immune serum 3. Anti-Calmegin antiserum
# Ikawa M., et al. Calmegin is required for fertilin alpha/beta heterodimerization and
sperm fertility. Dev Biol. 2001 Dec 1;240(1):254-61. PMID: 11784061
# Ikawa M., et al. Calsperin is a testis-specific chaperone required for sperm fertility.
J Biol Chem. 2011 Feb 18;286(7):5639-46. PMID: 21131354
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