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Anti Calmegin

Background

Calmegin plays an important role in sperm fertility. Binds calcium ions. Specifically expressed during male meiotic germ cell development.

Applications

 

Fig.1. Western blotting analysis of testis extracts of wild-type and knockout mice with anti-Calmegin antibody. 20 µg of Triton X-100 extracts from mouse testes was reacted with anti-Calmedin antiserum at 1/1,000 dilution. Arrow indicates the position of intact Calmegin.

 

Fig.2. Immunoprecipitation of Calmegin from mouse testis. One mg of testis lysate was incubated with 2 µl of anti-Calmegin antiserum and 50 µl. of protein-A conjugated magnetic beads (Miltenyi Biotec) and immunoprecipitated according to the protocol of supplier. The immunoprecipitated sample was analyzed by western blotting with the antibody at 1/1,000 dilution. 1. Input 2. non-immune serum 3. Anti-Calmegin antiserum

Product List

Product Name Cat# Quantity Price

Anti Calmegin

BAM-73-034-EX 100UL

¥ 34,500
$ 460
€ 345

References

# Ikawa M., et al. Calmegin is required for fertilin alpha/beta heterodimerization and
sperm fertility. Dev Biol. 2001 Dec 1;240(1):254-61. PMID: 11784061
# Ikawa M., et al. Calsperin is a testis-specific chaperone required for sperm fertility.
J Biol Chem. 2011 Feb 18;286(7):5639-46. PMID: 21131354

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.